Peptidomics as a Tool to Assess the Cleavage of Wine Haze Proteins by Peptidases from Drosophila suzukii Larvae

Author:

Albuquerque Wendell1ORCID,Ghezellou Parviz2ORCID,Lee Kwang-Zin3,Schneider Quintus1,Gross Phillip1,Kessel Tobias3,Omokungbe Bodunrin3,Spengler Bernhard2ORCID,Vilcinskas Andreas34ORCID,Zorn Holger13ORCID,Gand Martin1ORCID

Affiliation:

1. Institute of Food Chemistry and Food Biotechnology, Justus Liebig University Giessen, Heinrich-Buff-Ring 17, 35392 Giessen, Germany

2. Institute of Inorganic and Analytical Chemistry, Justus Liebig University Giessen, Heinrich-Buff-Ring 17, 35392 Giessen, Germany

3. Fraunhofer Institute for Molecular Biology and Applied Ecology, Ohlebergsweg 12, 35392 Giessen, Germany

4. Institute for Insect Biotechnology, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany

Abstract

Thermolabile grape berry proteins such as thaumatin-like proteins (TLPs) and chitinases (CHIs) promote haze formation in bottled wines if not properly fined. As a natural grapevine pest, the spotted-wing fly Drosophila suzukii is a promising source of peptidases that break down grape berry proteins because the larvae develop and feed inside mature berries. Therefore, we produced recombinant TLP and CHI as model thermolabile wine haze proteins and applied a peptidomics strategy to investigate whether D. suzukii larval peptidases were able to digest them under acidic conditions (pH 3.5), which are typically found in winemaking practices. The activity of the novel peptidases was confirmed by mass spectrometry, and cleavage sites within the wine haze proteins were visualized in 3D protein models. The combination of recombinant haze proteins and peptidomics provides a valuable screening tool to identify optimal peptidases suitable for clarification processes in the winemaking industry.

Funder

German Ministry of Economic Affairs and Climate Action

Deutsche Forschungsgemeinschaft

State of Hesse

LOEWE Center Insect Biotechnology and Bioresources

Publisher

MDPI AG

Subject

Molecular Biology,Biochemistry

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