Affiliation:
1. College of Veterinary Medicine, Sichuan Agricultural University, No. 211 Huimin Road, Wenjiang District, Chengdu 611130, China
Abstract
Eriodictyol occurs naturally in a variety of fruits and vegetables, and has drawn significant attention for its potential health benefits. This study aims to look into the effects of eriodictyol on acute liver injury (ALI) induced by LPS/D-GalN and elucidate its potential molecular biological mechanisms. A total of 47 targets were predicted for the treatment of ALI with eriodictyol, and the PI3K/AKT signaling pathway played a key role in the anti-ALI processing of this drug. The in vivo experiment showed that eriodictyol can effectively reduce liver function-related biochemical indicators such as ALT, AST, and AKP. Eriodictyol can also up-regulate the levels of SOD and GSH, and inhibit the release of IL-1β, IL-6, and TNF-α. Additionally, TUNEL staining, immunohistochemistry, and RT-PCR experiments showed that eriodictyol activated the PI3K/AKT pathway and decreased the expression of Bax, caspase3, and caspase8 while increasing the expression of Bcl-2 m-RNA. Finally, molecular docking experiments and molecular dynamics simulations confirmed the stable binding between eriodictyol and PI3K, AKT molecules. This study showed that eriodictyol can activate the PI3K/AKT signaling pathway to alleviate ALI-related oxidative stress and apoptosis.
Subject
Food Science,Nutrition and Dietetics
Reference34 articles.
1. p300/CBP inhibitor A-485 alleviates acute liver injury by regulating macrophage activation and polarization;Peng;Theranostics,2019
2. Pan, Y., Cao, M., You, D., Qin, G., and Liu, Z. (2019). Research Progress on the Animal Models of Drug-Induced Liver Injury: Current Status and Further Perspectives. BioMed Res. Int., 2019.
3. Drug-Induced Liver Toxicity and Prevention by Herbal Antioxidants: An Overview;Singh;Front. Physiol.,2015
4. Dioscin attenuates Bleomycin-Induced acute lung injury via inhibiting the inflammatory response in mice;Wu;Exp. Lung Res.,2019
5. Dioscin Exerts Protective Effects Against Crystalline Silica-induced Pulmonary Fibrosis in Mice;Li;Theranostics,2017
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