Abstract
Paclitaxel (Taxol®) is a potent anticancer agent, but the widespread pharmaceutical use of paclitaxel is hampered by its limited availability due to low accumulation levels in the native yew (Taxus spp.) plants. Currently, hairy root culture is an emerging biotechnological tool that presents several advantages such as reduced costs and higher specialized metabolite production, therefore, its application to paclitaxel production can be of commercial and medicinal interest. The objective of present study was to induce hairy root in Taxus baccata L. by transformation with the wild type Rhizobium rhizogenes A4 strain. Thus, T. baccata was inoculated by three different inoculation methods: (a) ex vitro seedlings inoculation by direct injection of a liquid bacterial culture; (b) ex vitro needles inoculation by liquid co-culturing with bacteria; (c) ex vitro shoots inoculation by dipping liquid bacterial culture. Hairy roots were formed only from ex vitro seedlings inoculated by the direct inoculation method, with transformation efficiency of 14.3%. Formation of hairy roots was observed two months after inoculation. This project forms the basis for the establishment of hairy root cultures from T. baccata for the production of paclitaxel.
Funder
China Scholarship Council
Independent Research Fund Denmark Technology and Production
Subject
Horticulture,Plant Science
Reference63 articles.
1. Taxus spp.: Botany, Horticulture, and Source of Anti-Cancer Compounds;DeLong;Hortic. Rev.,2010
2. Plant antitumor agents. VI. The Isolation and structure of taxol, a novel antileukemic and antitumor agent from Taxus brevifolia;Wani;J. Am. Chem. Soc.,1971
3. Chadwick, D.J., and Marsh, J. (2007). Ciba Foundation Symposium 185—Ethnobotany and the Search for New Drugs, Wiley.
4. When Taxol met tubulin;Arnst;J. Biol. Chem.,2020
5. Paclitaxel (Taxol);Rowinsky;N. Engl. J. Med.,1995
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