Changes in Morpho-Anatomical and Eco-Physiological Responses of Viburnum tinus L. var lucidum as Modulated by Sodium Chloride and Calcium Chloride Salinization

Abstract

Salinity in water and soil is among the major constraints to the cultivation of ornamental crops since it can affect their growth and aesthetic value. A greenhouse experiment was carried out to assess whether the application of two different salts (80 mM NaCl or 53.3 mM CaCl2, with a final ionic concentration of 160 mM) could differently modulate the anatomical and physiological acclimation of an important ornamental species such as Viburnum tinus L. var. lucidum. Eco-physiological analyses (e.g., leaf gas exchange and chlorophyll a fluorescence emission) were performed and leaves were subjected to light microscopy analysis to quantify functional anatomical traits through digital image analysis. Results showed that the two iso-osmotic solutions induced different structure-mediated physiological alterations in V. tinus plants. Photosynthesis was lowered by CaCl2 treatments (−58%) more than by NaCl (−37%), also due to the occurrence of photodamage apart from stomatal limitations. Neither Na+ nor Cl− exhibited toxic effects in leaf lamina structure which was reflected in the limited reduction in dry matter accumulation. Overall data were interpreted focusing on the coordination among leaf structural and functional traits suggesting that the fine control of functional anatomical traits contributes to physiological acclimation to both stressful conditions.