Chemotypes of Species of the Genus Thymus L. in Carpathians Region of Ukraine—Their Essential Oil Qualitative and Quantitative Characteristics and Antimicrobial Activity

Abstract

The study of the R&D in this paper is to determine the range of essential oils (EOs) in the raw materials of species of the genus Thymus of the natural flora in the Carpathian region and their antimicrobial activity. It was found that the component range of EO in species of the genus Thymus depends on the microclimatic conditions of the population. The range of essential oils in the raw material of Th. Serpyllum and Th. Pulegoides is 7–9 mL and Th. Marschallianus is 3.5 mL. The research found that the plants Th. Serpyllum and Th. Pulegoides that grow in sunny habitats have an aromatic mono- and bicyclic monoterpenoid chemotype (K/α-T-neol/ G/p-C/B), with total dominance of carvacrol and p-cymene. The populations of Th. Serpyllum, which grow on the edges of sparse pine forests, and populations of Th. Pullegioide, with denser plant cover and which grow in meadows, have an acyclic and bicyclic monoterpene chemotype (G/α-T-neol/B/K). Plants that grow in the communities of meadow-steppe vegetation have the following chemotypes: Th. Serpyllum—L/K/G with 63% of linalool, Th. Pullegioides—G/α-T-neol/L/B, and Th. Marschallianus—α-T-neol/K/L/α-T-nen/G/B. Of these, the dominant chemotypes are α-terpineol and carvacrol (28:6.5%). A wide spectrum of antimicrobial activity was registered in samples of Th. Pullegoides and Th. Serpyllum that have an aromatic-monoterpenoid chemotype. Essential oils of Th. Pullegoides were dominated by carvacrol, and p-cymene had the highest fungicidal action (41.00 ± 1.0%). Plant populations of Th. Pullegoides and Th. Serpyllum with the aromatic-monoterpenoid chemotype are suggested by R&D to be of use in the pharmaceutical industry. They have high contents of natural components, which are effective in a wide spectrum of antimicrobial activity. The EO of Th. Marschallianus had the lowest influence on the inhibition of bacterial and fungal reproduction.