Transcriptome Analysis on the Underlying Physiological Mechanism of Calcium and Magnesium Resolving “Sugar Receding” in ‘Feizixiao’ Litchi Pulp

Abstract

The sugar contents of ‘Feizixiao’ litchi (Litchi chinensis Sonn.) decrease at full maturity; calcium (Ca) and magnesium (Mg) foliar fertilizer can resolve this “sugar receding”. To investigate the physiological mechanism of Ca and Mg foliar fertilizer used to resolve the “sugar receding” phenomenon in ‘Feizixiao’ litchi pulp, 16-year-old litchi trees were treated with 0.3% CaCl2 + 0.3% MgCl2 foliar spraying or water as a control. We determined the pulp sugar content over a two-year period in 2020 and 2021. Pulp total RNA was extracted for transcriptome sequencing in 2020, and the expression pattern of 10 differentially expressed genes (DEGs) was verified by real-time PCR in 2020 and 2021. The results showed that the fertilizer treatment significantly increased pulp fructose and total soluble sugar contents at maturity in both years. According to Gene Ontology (GO) functional enrichment analysis, there were 155 DEGs divided into 35 GO categories, among which 49 DEGs were divided into 49 pathways according to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. We isolated sugar-metabolism-related enzyme genes, including sucrose synthase (SS), acid invertase (AI), neutral invertase (NI), sucrose phosphate synthase (SPS), and hexokinase (HK). All sucrose-metabolism-related enzyme (NI, AI, SS, SPS) genes were downregulated, and six of the seven HK genes were downregulated. The expression patterns of the 10 DEGs were verified by real-time PCR, which showed significant linear relationships (r2020 = 0.9127, r2021 = 0.8705). In conclusion, the fertilizer treatment inhibited the synthesis of sucrose and phosphorylation of hexose by downregulating the expression of the SS, SPS, and HK genes, thus increasing the fructose and total soluble sugar contents in ‘Feizixiao’ litchi.