Abstract
Creating high-quality and salt-tolerant plant germplasm is an effective strategy to improve the utilization of saline-alkali land. Salt-induced callus mutation was used to create dandelion germplasm and mutant dandelion calluses were obtained under NaCl concentrations of 0.8%, 1%, and 1.2% with the identification of random amplified polymorphic DNA (RAPD) markers. A new dandelion line, “Binpu 2”, selected from the progenies of dandelion tissue culture plantlets that originated from callus treated under 0.8% NaCl, was evaluated in light of its morphological characteristics, bioactive components, and antitumor functions. Results showed that the plant shape of “Binpu 2”was nearly upright; its cichoric acid content was 6.7 mg/g, which was 39.6% and 36.7% higher than its mother plant and local dandelion cultivar, respectively; its leaf water extracts of 0.2 g/mL had a significant inhibitory effect on cell polarity disruption-induced cell migration without affecting drosophila normal growth, revealed by the strong inhibitory effect on tumor cell migration, the increased level of MMP1 and β-Integrin, and the reduced E-cadherin expression. Our results suggested that “Binpu 2” originated from salt-induced mutant performed better than its mother plant and processed strong antitumor function, which was suitable for amplified cultivation in saline-alkali land for food and medicinal industrial development.
Funder
Hebei S & T Innovation Team of Modern Seed Industry of Traditional Chinese Medicine
HAAFS Science and Technology Innovation Special Project
Hebei Natural Science Foundation
Basic Research Funds of Hebei Academy of Agriculture and Forestry Sciences
Tangshan Talent Funding Project
Subject
Horticulture,Plant Science
Cited by
1 articles.
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