Application of Encapsulation Technology: In Vitro Screening of Two Ficus carica L. Genotypes under Different NaCl Concentrations
-
Published:2023-12-16
Issue:12
Volume:9
Page:1344
-
ISSN:2311-7524
-
Container-title:Horticulturae
-
language:en
-
Short-container-title:Horticulturae
Author:
Granata Irene1ORCID, Regni Luca2ORCID, Micheli Maurizio2ORCID, Silvestri Cristian3ORCID, Germanà Maria Antonietta1
Affiliation:
1. Department of Agricultural, Food and Forestry Sciences (SAAF), University of Palermo, Viale delle Scienze, Ed. 4, 90128 Palermo, Italy 2. Department of Agricultural, Food and Environmental Sciences, University of Perugia, Borgo XX Giugno 74, 06121 Perugia, Italy 3. Department of Agriculture and Forest Sciences, University of Tuscia, Via San Camillo de Lellis snc, 01100 Viterbo, Italy
Abstract
Salinity stress represents an increasing issue for agriculture and has a great negative impact on plant growth and crop production. The selection of genotypes able to tolerate salt stress could be a suitable solution to overcome the problem. In this context, in vitro cultures can represent a tool for identifying the NaCl tolerant genotypes and quickly producing large populations of them. The possibility of exerting selection for tolerance to NaCl by using encapsulation technology was investigated in two genotypes of fig: ‘Houmairi’ and ‘Palazzo’. The effects of five concentrations of NaCl (0, 50, 100, 150 and 200 mM) added to the artificial endosperm were tested on the conversion of synthetic seeds and on the growth of derived shoots/plantlets. Moreover, proline (Pro) and malondialdehyde (MDA), the enzymatic activities of catalase (CAT), guaiacol peroxidase (POD), and EL (Electrolytic Leakage), as well as the chlorophyll content, flavanols, anthocyanins, and Nitrogen Balance Index (NBI) were determined on shoots/plantlet. The obtained results clearly showed that ‘Houmairi’ and ‘Palazzo’ could tolerate salt stress, although a strong difference was found depending on each specific physiological pathway. Indeed, ‘Houmairi’ was revealed to be more tolerant than ‘Palazzo’, with different response mechanisms to salt stress. The use of encapsulated vitro-derived explants proved to be a useful method to validate the selection of genotypes tolerant to salinity stress. Further investigation in the field must validate and confirm the legitimacy of the approach.
Subject
Horticulture,Plant Science
Reference54 articles.
1. Effect of Salinity on Physiological Processes in Plants;Giri;Microorganisms in Saline Environments: Strategies and Functions,2019 2. Global Mapping of Soil Salinity Change;Ivushkin;Remote Sens. Environ.,2019 3. Hussain, S., Shaukat, M., Ashraf, M., Zhu, C., Jin, Q., Zhang, J., Hussain, S., Shaukat, M., Ashraf, M., and Zhu, C. (2019). Climate Change and Agriculture, IntechOpen. 4. Adaptation of Plants to Salinity;Shannon;Advances in Agronomy,1997 5. A Review: Impact of Salinity on Plant Growth;Safdar;Nat. Sci.,2019
|
|