Affiliation:
1. Department of Vegetable Science, College of Horticulture, China Agricultural University, Beijing 100193, China
Abstract
Bacterial canker of tomato is a systemic disease caused by Clavibacter michiganensis (Cm), which poses a grave threat to tomato production worldwide. Towards the identification of genes underlying resistance to Cm infection, the transcriptome of the resistant inbred backcross line IBL2353 carrying the Rcm2.0 locus derived from Solanum habrochaites LA407 and the susceptible Solanum lycopersicum line Ohio88119 was comparatively analyzed after Cm inoculation, and the analysis focused on the genes with different expression patterns between resistant and susceptible lines. Gene ontology (GO) analysis revealed that top terms of differentially expressed genes comprised ubiquitin protein ligases, transcription factors, and receptor kinases. Then we screened out some genes which are potentially associated with the defense response against Cm infection in IBL2353 including the wall-associated receptor kinase-like 20 (WAKL20), and virus-induced gene silencing showed it contributes resistance to Cm infection. In addition to Cm-induced genes related to resistance, the expression of eight homologs from six susceptibility (S) gene families was analyzed. These putative resistance and susceptibility genes are valuable resources for molecular resistance breeding and contribute to the development of new control methods in tomato.
Funder
National Natural Science Foundation of China
Subject
Horticulture,Plant Science
Reference97 articles.
1. Re-classification of Clavibacter michiganensis subspecies on the basis of whole-genome and multi-locus sequence analyses;Li;Int. J. Syst. Evol. Micr.,2018
2. Bacterial canker of tomato: Revisiting a global and economically damaging seedborne pathogen;Tancos;Plant Dis.,2021
3. Wang, Y., Deng, S., Li, Z., and Yang, W. (2022). Advances in the characterization of the mechanism underlying bacterial canker development and tomato plant resistance. Horticulturae, 8.
4. A new tomato disease of economic importance;Smith;Science,1910
5. EPPO (2022, October 11). Distribution of Clavibacter michiganesis subsp. michiganesis. Available online: https://gd.eppo.int/taxon/CORBMI/distribution.
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献