Application and Expansion of Virus-Induced Gene Silencing for Functional Studies in Vegetables

Author:

Wang Zheng12,Cao Shoujun3,Xu Xinyang2,He Yanjun2ORCID,Shou Weisong2,Munaiz Eduardo D.4ORCID,Yu Chao1ORCID,Shen Jia2ORCID

Affiliation:

1. College of Horticulture Science, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China

2. Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

3. Yantai Agricultural Science Academy of Shandong Province, Yantai 265500, China

4. UniLaSalle, AGHYLE UP 2018.C101, 60000 Beauvais, France

Abstract

Increased consumption of vegetables has been recommended worldwide as a part of a healthy diet; therefore, determining gene function among breeding materials is crucial for vegetable improvement to meet the sustainable development of new vegetable varieties. However, genetic transformation is time-consuming and laborious, which limits the exploration of gene function for various vegetable crops. Virus-Induced Gene Silencing (VIGS) can perform large-scale and rapid gene silencing in plants due to a reduction in the experimental period and its independence from the stable genetic transformation, providing an excellent opportunity for functional research. VIGS can accelerate model plant research and make it easier to analyze gene function and validation in vegetable crops. Moreover, with the advent of technologies such as virus-mediated heterologous protein expression and the development of CRISPR/Cas9 technology, virus-mediated genetic tools have ushered in a new era in genetics and crop improvement. This study summarizes recent achievements in VIGS and Virus-Induced Gene Editing (VIGE) in vegetables. We also identify several challenges in the current state of VIGS technology in vegetables, serving as a guide for future research.

Funder

Department of Science and Technology of Zhejiang Province

Key Research and Development Project of Zhejiang Province

Major Science and Technology Project of Plant Breeding in Zhejiang Province

Publisher

MDPI AG

Subject

Horticulture,Plant Science

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