Self-Incompatibility of Camellia weiningensis Y.K. Li.

Abstract

This study compared the pollen tube growth, fruit setting, and seed setting characteristics of Camellia weiningensis Y.K. Li. under self- and cross-pollination to identify its self-incompatibility characteristics and types. C. weiningensis pollen tube growth was observed by fluorescence and scanning electron microscopy, and a field experiment with manual pollination verified fruit and seed setting characteristics. Both self- and cross-pollinated pollen germinated from the stigma. At 72 h after cross-pollination, the pollen tube reached the style base, with tube growth showing a slow-fast-slow pattern. The tube growth speed was maximal, 343.36 μm·h−1, at 12–24 h after pollination. For self-pollination, the pollen did not germinate on the stigma 4 h before pollination. At 12–24 h after pollination, the growth rate was maximal at 263.36 μm·h−1. At 96 h, a small amount of pollen reached the style base and stagnated. The pollen tube end showed callose reactions, such as abnormal swelling, distortion, and brightness. In the field experiment, the fruit setting rate under cross-pollination was 68.5%, while that under self-pollination was 15.3%. When the fruit grew to maturity, the growth dynamics of the transverse and longitudinal diameters showed a “slow-fast-slow”, S-shaped curve. The number of aborted selfed and outcrossed seeds was 13.9 and 4.7, respectively. Thus, C. weiningensis showed self-incompatibility. The self-incompatibility reaction occurred at the style base and represented prezygotic self-incompatibility. The self-incompatibility of C. weiningensis is one of the main reasons for its low seed setting rate, which should be fully considered in cross breeding.