“In Vitro” Ovule Culture to Improve Genetic Variability in Hydrangea macrophylla

Author:

Nesi Beatrice1,Ghiselli Lisetta12ORCID,Gori Massimo23ORCID,Natale Roberto3ORCID,Tomiozzo Regina24ORCID,Mansuino Andrea5,Biricolti Stefano2ORCID

Affiliation:

1. CREA Research Center for Vegetable and Ornamental Crops (CREA OF), Via dei Fiori, 8, 51017 Pescia, Italy

2. Department of Agriculture, Food, Environment and Forestry (DAGRI), University of Florence, Piazzale delle Cascine, 18, 50144 Firenze, Italy

3. Centro Interdipartimentale di Servizi per le Biotecnologie di interesse Agrario, Chimico, Industriale (CIBIACI), University of Florence, Via Romana, 21, 50125 Firenze, Italy

4. Departament of Plant Science, Federal University of Santa Maria (UFSM), Avenida Roraima, 1000, Santa Maria 97105-900, Brazil

5. Azienda Mansuino, Strada Villetta, 42, 18038 Sanremo, Italy

Abstract

In flowering plants, such as Hydrangea macrophylla, the main breeding objective is to increase genetic variability in ornamental traits. This study investigates in vitro techniques, through ovule culture, to overcome the hybridization barriers and increase the efficiency of crossing in Hydrangea macrophylla in which breeding has been hampered by a fairly long breeding cycle and lack of information about its genetic resources. Two different types of media were compared, Gamborg B5 and Murashige and Skoog basal salts, to verify the germination rate of immature ovules in different intraspecific crosses. The germination rate and viability of the seedlings were influenced by the parental genotypes in the different combinations of crossing, highlighting, in some cases, the poor compatibility between some of them. The crossing combination “Parental A × Parental B”, showed the highest germinated ovules percentage (78.3%). The media used seem to less affect the ovule germination while mainly influencing the development and growth of the young seedlings and in particular the number of leaves, the branching attitude, and root length, with the Gamborg medium determining up to a 30% increase, compared to MS medium. In addition, we tested the effectiveness of using SSR markers to assess the parentage of the putative hybrids even though only three out of twelve SSR markers showed allelism. Although the number of SSR markers was low, they were allowed to profile the parentage according to Mendelian laws.

Funder

Mansuino Company

Publisher

MDPI AG

Subject

Horticulture,Plant Science

Reference33 articles.

1. A monograph of the genus Hydrangea;McClintock;Proc. Calif. Acad. Sci.,1957

2. Verification and establishment of H. macrophylla ‘Kardinal’ × H. paniculata ‘Brussels Lace’ interspecific hybrids;Reed;J. Environ. Hort.,2001

3. Reed, S.M. (2004, January 13). Floral characteristics of a Hydrangea macrophylla × H. paniculata hybrid. Proceedings of the Southern Nursery Association Research Conference, Atlanta, GA, USA.

4. Genetic diversity estimates for the gens Hydrangea and development of a molecular key based on SSRs;Rinehart;J. Amer. Soc. Hort. Sci.,2006

5. An update on Hydrangea macrophylla breeding targets and genomics;Rinehart;Acta Hort.,2018

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