Effects of Different Media and Their Strengths in In Vitro Culture of Three Different Cistus creticus L. Populations and Their Genetic Assessment Using Simple Sequence Repeat Molecular Markers-Reference-Cited by-同舟云学术

Effects of Different Media and Their Strengths in In Vitro Culture of Three Different Cistus creticus L. Populations and Their Genetic Assessment Using Simple Sequence Repeat Molecular Markers

Published:2024-01-22 Issue:1 Volume:10 Page:104
ISSN:2311-7524
Container-title:Horticulturae
language:en
Short-container-title:Horticulturae

Author:

Ioannidis Kostas¹^ORCID,Koropouli Polyxeni²

Affiliation:

1. Laboratory of Forest Genetics and Biotechnology, Institute of Mediterranean and Forest Ecosystems, Hellenic Agricultural Organization “Demeter”, Ilissia, 11528 Athens, Greece

2. Ministry of Education, 15180 Athens, Greece

Abstract

Cistus creticus L. (rockrose), a species of ecological and medicinal significance, constitutes a valuable component of the Mediterranean ecosystem. The present study investigated the effect of the inorganic salt concentration of Murashige and Skoog medium (MS), woody plant medium (WPM), and Driver and Kuniyaki Walnut medium (DKW) at several strengths (1/8×, 1/4×, 1/2×, 1×, and 2×) on the in vitro growth and organogenesis of rockrose. Significant interactions were observed throughout the experiments between pairs of plant origins, medium types, and strengths, and we also examined the extent to which they affected the studied traits was examined. The types of nutrient medium affected all studied traits except shoot and root percentages. The maximum growth percentage (143.49%) was gained using full-strength WPM. The best performance in shoot percentage was obtained using MS (100%) at several strengths along with 1× WPM (100%). The topmost rooting percentage values (98.61%) were obtained using 1× WPM and 1/2× DKW. The highest number of shoots and roots were observed using full-strength MS (9.39) and half-strength WPM (6.49), respectively. The maximum values for shoot and root length were achieved using 1/2× MS (0.78 cm) and 1/8× WPM (1.55 cm), respectively. The origin of the plant material did not influence any studied trait. Moreover, the genetic relations among the populations used in the in vitro culture were assessed using simple sequence repeats (SSR) markers. Twenty-eight alleles were identified across all five STR loci. The different and effective alleles per locus were 5.60 and 4.72, respectively. The average observed and expected heterozygosity was estimated at 0.52 and 0.72, respectively. Shannon’s information index and the inbreeding coefficient (F) were assessed at 1.48 and 0.30, respectively, revealing a narrow genetic base and high genetic similarity among origins, suggesting that they belong to the same population.

Publisher

MDPI AG

Subject

Horticulture,Plant Science

Link

https://www.mdpi.com/2311-7524/10/1/104/pdf

Reference91 articles.

1. In Vitro Propagation of Cistus creticus L.;Zygomala;Acta Hortic.,2003

2. Trabaud, L. (1987). The Role of Fire in Ecological Systems, SPBAcademic Publishing.

3. Le Houérou, H.N. (1973, January 22–23). Fire and Vegetation in the Mediterranean Basin. Proceedings of the 13th Annual Tall Timbers Fire Ecology Conference, Talahassee, FL, USA.

4. Heywood, V. (1978). Flowering Plants of the World, Oxford University Press.

5. Tutin, T.G., Heywood, V.H., Burges, N.A., Valentine, D.H., Walters, S.M., and Webb, D.A. (1968). Flora Europaea: Cistaceae, Cambridge University Press.