Differential Tolerance of Primary Metabolism of Annona emarginata (Schltdl.) H. Rainer to Water Stress Modulates Alkaloid Production

Author:

Honório Ana Beatriz Marques1ORCID,De-la-Cruz-Chacón Ivan2ORCID,da Silva Gustavo Cabral1ORCID,Mimi Carolina Ovile1ORCID,Campos Felipe Girotto1ORCID,da Silva Magali Ribeiro3ORCID,Boaro Carmen Silvia Fernandes1ORCID,Ferreira Gisela1ORCID

Affiliation:

1. Department of Biodiversity and Biostatistics, Institute of Biosciences, São Paulo State University (Unesp), Prof. Dr. Antônio Celso Wagner Zanin Street, 250, Botucatu 18618-689, SP, Brazil

2. Laboratorio de Fisiología y Química Vegetal, Instituto de Ciencias Biológicas, Universidad de Ciencias y Artes de Chiapas, Libramiento Norte-Poniente 1150, Tuxtla Gutiérrez 29039, CHIS, Mexico

3. Forest, Soil and Environmental Sciences Department, Faculty of Agronomic Science, São Paulo State University, UNESP, Avenida Universitária 3780, Botucatu 18610-034, SP, Brazil

Abstract

Annona emarginata produces alkaloids of ecological and pharmacological interest and is tolerant to water and biotic stress, so it is used as rootstock for other Annonaceae fruits. There are few reports in the literature on how contrasting water stress impacts the production of specialized metabolites in Annonaceae and how primary metabolism adjusts to support such production. The objective of this investigation was to evaluate how drought and flooding stress affect alkaloid concentration and the primary metabolism of young A. emarginata plants. Three water levels (flooding, field capacity, and drought) were studied at two moments (stress and recovery). Variables analyzed were gas exchange levels, chlorophyll a fluorescence, leaf sugars, total alkaloid content, alkaloid profile, and Liriodenine concentration. The photosynthetic metabolism of A. emarginata was affected by water stress, with plants having a greater ability to adapt to drought conditions than to flooding. During the drought, a reduction in photosynthetic efficiency with subsequent recovery, higher starch and trehalose concentrations in leaves, and total alkaloids in roots (480 µg.g−1) were observed. Under flooding, there was a reduction in photochemical efficiency during stress, indicating damage to the photosynthetic apparatus, without reversal during the recovery period, as well as a higher concentration of total sugars, reducing sugars, sucrose, glucose, and fructose in leaves, and Liriodenine in roots (100 µg.g−1), with a lower concentration of total alkaloids (90 µg.g−1). It could be concluded that there is differential tolerance of A. emarginata to water stress, inducing the modulation of alkaloid production, while drought promotes a higher concentration of total alkaloids and flooding leads to an increase in the Liriodenine concentration.

Funder

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code

CAPESPrint—Unesp and National Council for Scientific and Technological Development

Publisher

MDPI AG

Reference81 articles.

1. The Natural History of Annonaceae;Chatrou;Bot. J. Linn. Soc.,2012

2. Phylogenomics of the Major Tropical Plant Family Annonaceae Using Targeted Enrichment of Nuclear Genes;Couvreur;Front. Plant Sci.,2019

3. Mendes-Silva, I., Lopes, J.C., Silva, L.V., and Bazante, M.L. (2023, July 20). Annona in Flora Do Brasil 2020, Available online: https://floradobrasil2020.jbrj.gov.br/reflora/floradobrasil/FB110243.

4. Annonnaceae from Central-Eastern Brazil;Maas;Rodriguésia,2001

5. A Linear Sequence to Facilitate Curation of Herbarium Specimens of Annonaceae;Chatrou;Kew Bull.,2018

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3