Short-Term Liquid Nitrogen Storage of Pyrostegia venusta Embryos: Effects on Germination, Phenotypic and Biochemical Characteristics, and In Vitro Secondary Metabolite Production

Author:

Coimbra Mairon César1ORCID,Garcia Israel José Pereira2,Santos Hérica de Lima2,Castro Ana Hortência Fonsêca1ORCID

Affiliation:

1. Laboratory of Botany and Medicinal Plants, Postgraduate Program in Biotechnology, Universidade Federal de São João del-Rei (UFSJ), Campus Centro-Oeste Dona Lindu, Avenida Sebastião Gonçalves Coelho, 400—Chanadour, Divinópolis 35501-296, MG, Brazil

2. Laboratory of Cellular Biochemistry, Postgraduate Program in Health Sciences, Universidade Federal de São João del-Rei (UFSJ), Campus Centro-Oeste Dona Lindu, Avenida Sebastião Gonçalves Coelho, 400—Chanadour, Divinópolis 35501-296, MG, Brazil

Abstract

In this study, short-term liquid nitrogen (LN) storage was used as a strategy to conserve Pyrostegia venusta embryos, and its effects on in vitro germination, phenotypic and biochemical characteristics, and in vitro secondary metabolite production were assessed. Embryos stored in LN for 1 and 7 days presented a higher germination rate and germination speed index compared to those of the control (non-cryostored embryos). Short-term LN storage also favored the phenotypic characteristics of seedlings. LN storage significantly affected the proteins (PTN), soluble sugar (SS) and reducing sugar (RS) contents, oxidative metabolism, and phenylalanine ammonia-lyase (PAL) activity, as well as the total phenolic compound, flavonoid, phytosterol, and alkaloid levels in seedlings regenerated from embryos cryostored for 7 days. Benzoic acid derivatives and flavonoids were observed in regenerated non-acclimatized seedlings. LN storage did not affect the survival rate or phenotypic characteristics of seedlings during acclimatization. Acclimatization promoted significant changes in PTN, SS and RS contents, oxidative metabolism, and PAL activity in seedlings from embryos cryostored for 7 days. Roots from acclimatized seedlings exhibited the highest phenolic, phytosterol, and total alkaloid levels. Differences in the chromatographic profiles of the acclimatized seedlings compared with the non-acclimatized seedlings were observed. LN storage can be an effective means of ex situ conservation of P. venusta genetic resources.

Funder

Conselho Nacional de Desenvolvimento Científico e Tecnológico

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil

Publisher

MDPI AG

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