Affiliation:
1. Shandong Institute of Pomology, Longtan Road No. 66, Tai’an 271000, China
2. Huaiyin Institute of Technology, Meicheng East Road No. 1, Huaian 223001, China
3. National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Zhihui Road No. 8, Dongying 257335, China
Abstract
The “Qixiadaxiangshui” pear (Pyrus bretschneideri Rehd.1) is a highly valued cultivar known for its crisp texture, abundant juice, and rich aroma. In this study, we reported the first complete chloroplast genome sequence of the “Qixiadaxiangshui” pear, which is 159,885 bp in length with a GC content of 36.58%. The genome exhibits a typical circular quadripartite structure, comprising a large single-copy region (LSC), a small single-copy region (SSC), and a pair of inverted repeat regions (IRs). A total of 131 genes were identified, including 84 protein-coding genes, 8 rRNA genes, and 37 tRNA genes. We also identified 209 simple sequence repeats (SSRs) and several mutation hotspots, such as ndhC-trnM-CAU and trnR-UCU-atpA, which can be applied in molecular identification and phylogenetic studies of Pyrus. Comparative genomic analysis showed high conservation among ten pear cultivars. Phylogenetic analysis indicated that the “Qixiadaxiangshui” pear is closely related to germplasm Dangshansuli, Wonwhang, and Yali, suggesting a recent common ancestor. These findings provided valuable insights into the genetic diversity and evolutionary dynamics of the Pyrus species and contribute to the conservation and breeding of pear germplasm resources.
Funder
National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land
earmarked fund for China Agriculture Research System
Natural Science Foundation of Shandong Province, China
Agricultural Science and Technology Innovation Project of Shandong Academy of Agricultural Sciences, China
Youth Foundation of Shandong Institute of Pomology, China
Science and Technology Innovation Project of Shandong Land Group Dongying Co., Ltd.