Reference Gene Selection and Gene Expression Analysis during Gall Development of Zizania latifolia-Reference-Cited by-同舟云学术

Reference Gene Selection and Gene Expression Analysis during Gall Development of Zizania latifolia

Published:2024-07-18 Issue:7 Volume:10 Page:759
ISSN:2311-7524
Container-title:Horticulturae
language:en
Short-container-title:Horticulturae

Author:

Li Yipeng¹^ORCID,Yi Huan²,Gu Qing²^ORCID,Zheng Zhaisheng¹,Zhu Mingxing¹,Zha Xiaojun²,Zhang Shangfa¹,Yang Mengfei¹^ORCID

Affiliation:

1. Zhejiang Provincial Key Laboratory of Characteristic Aquatic Vegetable Breeding and Cultivation, Jinhua Academy of Agricultural Sciences, Jinhua 321051, China

2. College of Life Sciences, Zhejiang Normal University, Jinhua 321001, China

Abstract

The stem tips of Zizania latifolia at different development stages were used as research materials. The expression stability of nine candidate reference genes (ACT1, H2B, UBI, EF-1α, GAPDH, β-actin, 60S, SKIP and AQP) were evaluated using qRT-PCR. The data were analyzed with GeNorm and NormFinder software. Present results indicated that the expression of ACT1 was stable and that it could be used as the optimal reference gene for studying the development stage of gall formation. ACT1 was selected as the reference gene to verify the expression level of the correlative genes in the gall formation stage of Z. latifolia. Our results were consistent with the previous transcriptome sequencing results. This study revealed that ACT1 was the classic reference gene for the analysis of correlative genes in all of the gall development stages of Z. latifolia.

Funder

earmarked fund for CARS

Zhejiang Provincial Major Agricultural Science and Technology Projects of New Varieties Breeding

Jinhua Science and Technology Program

Publisher

MDPI AG

Link

https://www.mdpi.com/2311-7524/10/7/759/pdf

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