Phytoene Desaturase (PDS) Gene-Derived Markers Identify “A” and “B” Genomes in Banana (Musa spp.)-Reference-Cited by-同舟云学术

Phytoene Desaturase (PDS) Gene-Derived Markers Identify “A” and “B” Genomes in Banana (Musa spp.)

Published:2024-03-19 Issue:3 Volume:10 Page:294
ISSN:2311-7524
Container-title:Horticulturae
language:en
Short-container-title:Horticulturae

Author:

Nascimento Fernanda dos Santos¹^ORCID,Mascarenhas Marcelly Santana¹^ORCID,Boaventura Samantha Costa²,de Souza Carla Catharina Hora²,de Souza Ramos Andresa Priscila³,de Jesus Rocha Anelita¹^ORCID,da Silva Soares Julianna Matos¹^ORCID,Diniz Leandro Eugenio Cardamone⁴^ORCID,de Oliveira Mendes Tiago Antônio⁵,Ferreira Claudia Fortes³^ORCID,Amorim Edson Perito³^ORCID

Affiliation:

1. Post-Graduate Program in Biotechnology, State University of Feira de Santana, Avenida Transnordestina, Novo Horizonte, Feira de Santana 44036-900, Bahia, Brazil

2. Center for Agricultural, Environmental and Biological Sciences, Federal University of Recôncavo da Bahia, Rua Rui Barbosa, 710, Centro, Cruz das Almas 44380-000, Bahia, Brazil

3. Embrapa Mandioca e Fruticultura, Rua Embrapa, s/n, Cruz das Almas 44380-000, Bahia, Brazil

4. Embrapa Soja, Rodovia Carlos João Strass, Londrina 86085-981, Paraná, Brazil

5. Department of Biochemistry and Molecular Biology, Federal University of Viçosa, Avenida Peter Henry Rolfs, s/n, Campus Universitário, Viçosa 36570-900, Minas Gerais, Brazil

Abstract

Phytoene desaturase (PDS) is a plant enzyme involved in carotenoid biosynthesis. The PDS gene has been used as a selective marker for genome editing in several plant species, including banana (Musa spp.). Its knockout promotes dwarfism and albinism, characteristics that are easily recognizable and highly favorable. In Musa spp., the A genome increases fruit production and quality, whereas the B genome is associated with tolerance to biotic and abiotic stresses. The objective of this study was to identify a molecular marker in the PDS gene to easily discriminate the A and B genomes of banana. A 2166 bp fragment for the “PDSMa” marker was identified as polymorphic for the A genome (identification accuracy of 99.33%), whereas ~332 and ~225 bp fragments were detected for the “PDSMb” marker with 100% accuracy using MedCalc software. In this study, we used genotypes with A and B genomes that are used in the genetic improvement of bananas and an accession with the BT genome. It was not possible to differentiate the accession with the BT genome from the others, suggesting that the markers do not have the capacity to separate the T genome from the A and B genomes. To the best of our knowledge, this is the first study to use the PDS gene to determine doses of the A genome and identify the B genome in Musa spp., which will aid in evaluating the genomic constitution of banana hybrids and accessions at the seedling stage and accelerating their classification in crop genetic improvement programs.

Funder

IITA/The Bill

Melinda Gates Foundation—Accelerated Breeding of Better Bananas

Publisher

MDPI AG

Link

https://www.mdpi.com/2311-7524/10/3/294/pdf

Reference46 articles.

1. FAOSTAT (2023, August 10). Food and Agriculture Organization of the United Nations. Available online: http://www.fao.org/faostat/en/#home.

2. The taxonomy and origins of the cultivated bananas;Simmonds;J. Linean Soc. Lond.,1955

3. The banana (Musa acuminata) genome and the evolution of monocotyledonous plants;Denoeud;Nature,2012

4. Martin, G., Baurens, F.C., Droc, G., Rouard, M., Cenci, A., Kilian, A., Hastie, A., Doležel, J., Aury, J.M., and Alberti, A. (2016). Improvement of the banana “Musa acuminata” reference sequence using NGS data and semi-automated bioinformatics methods. BMC Genom., 17.

5. Davey, M.W., Gudimella, R., Harikrishna, J.A., Sin, L.W., Khalid, N., and Keulemans, J. (2013). A draft Musa balbisiana genome sequence for molecular genetics in polyploid, inter-and intra-specific Musa hybrids. BMC Genom., 14.