Affiliation:
1. College of Horticulture, Henan Agricultural University, Zhengzhou 450046, China
2. Henan Province International Joint Laboratory of Horticultural Plant Biology, Henan Agricultural University, Zhengzhou 450046, China
3. College of Forestry, Nanjing Forestry University, Nanjing 210037, China
Abstract
The interaction between miRNAs (microRNAs) and target genes plays an important role in plant pistil development. MiRNAs related to pistils were explored in pomegranate. The differentially expressed miRNAs were screened at different developmental stages of pomegranate pistils, and their target differentially expressed mRNAs were further identified to clarify the regulatory effect of miRNAs on pistil development. In our study, 61 conserved miRNAs were identified in 30 families, including miR395, miR394, miR393, miR161, miR162, and miR168. Among them, miR156, miR157, miR159, miR160, miR164, miR165, miR166, miR167, miR169, and miR172 were involved in the development of flower organs. Eight miRNAs were randomly selected and verified for qRT-PCR analysis. The result analysis indicated that miR160, miR164, and miR172 might be positive factors in the regulation of pomegranate pistil development. MiR156 and miR166 might be involved in regulation of pomegranate pistil development as negative factors.
Funder
modern agricultural industrial technology systems of Henan province
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