Exploring MicroRNAs Associated with Pomegranate Pistil Development: An Identification and Analysis Study

Author:

Zhao Yujie12,Huang Jingyi3,Li Ming12,Ren Hongfang3,Jiao Jian12,Wan Ran12ORCID,Liu Yu12,Wang Miaomiao12ORCID,Shi Jiangli12ORCID,Zhang Kunxi12ORCID,Hao Pengbo12,Song Shangwei12,Bai Tuanhui12ORCID,Zheng Xianbo12

Affiliation:

1. College of Horticulture, Henan Agricultural University, Zhengzhou 450046, China

2. Henan Province International Joint Laboratory of Horticultural Plant Biology, Henan Agricultural University, Zhengzhou 450046, China

3. College of Forestry, Nanjing Forestry University, Nanjing 210037, China

Abstract

The interaction between miRNAs (microRNAs) and target genes plays an important role in plant pistil development. MiRNAs related to pistils were explored in pomegranate. The differentially expressed miRNAs were screened at different developmental stages of pomegranate pistils, and their target differentially expressed mRNAs were further identified to clarify the regulatory effect of miRNAs on pistil development. In our study, 61 conserved miRNAs were identified in 30 families, including miR395, miR394, miR393, miR161, miR162, and miR168. Among them, miR156, miR157, miR159, miR160, miR164, miR165, miR166, miR167, miR169, and miR172 were involved in the development of flower organs. Eight miRNAs were randomly selected and verified for qRT-PCR analysis. The result analysis indicated that miR160, miR164, and miR172 might be positive factors in the regulation of pomegranate pistil development. MiR156 and miR166 might be involved in regulation of pomegranate pistil development as negative factors.

Funder

modern agricultural industrial technology systems of Henan province

Publisher

MDPI AG

Subject

Horticulture,Plant Science

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3