Tolerance Screening for Phytophthora capsici and Fusarium solani f. sp. cucurbitae in Cucurbita spp. and Gene Expression Responses in Mutant Families

Abstract

Cucurbita species can be affected by soil-borne pathogens, such as Phytopthora capsici and Fusarium solani f. sp. cucurbitae (Fsc). Diverse commercial and conserved lines of Cucurbita spp. were tested. C. pepo subsp. pepo genotypes showed the highest susceptibility to both pathogens. The tolerance to P. capsici and Fsc was then screened in a zucchini mutant population. Two M3 mutant lines (Cp107 and Cp116) with a high occurrence of tolerant individuals to Phytophthora capsici were obtained from a screening of 160 M2 mutant lines. The M3 lines presented higher tolerance than the background MUCU-16. Furthermore, in the inoculated samples, both mutants overexpressed CpDEF and expressed more CpPAL and CpChiIV than the susceptible control. It has been previously shown that this expression pattern could be associated with tolerance in the P. capsici - Cucurbita spp. pathosystem. The M3 lines obtained could be applied in breeding programs, as they are likely to be compatible with the highly susceptible C. pepo subsp. pepo genotype.