Genotypic and Sanitary Characterization of Minority Grapevine Varieties Prospected in Andalusia, Spain

Author:

Rodríguez-Torres Inmaculada1,Martín Carrillo Ana2,Ramírez María del Pilar3,Gómez Gálvez Francisco Jesus4ORCID,Velasco Arjona Leonardo5ORCID,Padilla Carlos6,Cretazzo Enrico2ORCID

Affiliation:

1. Agroindustry and Food Quality Area, IFAPA Rancho de la Merced Center, Ctra. Cañada de la Loba PK 3,1, 11471 Jerez de la Frontera, Spain

2. Biotechnology and Plant Breeding Area, IFAPA Rancho de la Merced Center, Ctra. Cañada de la Loba PK 3,1, 11471 Jerez de la Frontera, Spain

3. Agroalimentary Technologies and Engineering, IFAPA Cabra Center, Antigua Ctra. Cabra-Doña Mencía, Km. 2,5, 14940 Cabra, Spain

4. Biotechnology and Plant Breeding Area, IFAPA Alameda del Obispo Center, Avda. Menéndez Pidal s/n, 14080 Cordoba, Spain

5. Sustainable Plant Protection Area, IFAPA Málaga Center, Cortijo de la Cruz s/n, 29140 Malaga, Spain

6. Plant Protection Department, IMIDA, Calle Mayor s/n, 30150 Murcia, Spain

Abstract

Andalusia is a Spanish region that is home to numerous minority varieties due to its diversity and territorial extension, offering the local viticulture the possibility of diversifying its wine production. The genotypic characterization of 98 specimens from six areas with a winemaking tradition in Andalusia was carried out between the years 2020 and 2022, by means of thirteen microsatellite markers, including the nine recommended by the OIV. A total of 33 different genotypes were obtained, 20 of which corresponded to profiles of already described varieties (11 of them are of 6 minority cultivars in Andalusia: ‘Rojal Tinto’, ‘Beba’, ‘Zurieles’, ‘Rome’, ‘Hebén’, ‘Mollar Cano’, ‘Listán Prieto’, ‘Listán del Condado’, ‘Jarrosuelto’, ‘Negra Dorada’, and ‘Mantúo de Pilas’), while the other 12 profiles did not match with previously identified varieties. These profiles were registered in the database of the IFAPA “Rancho de la Merced” Germplasm Bank. The eco-geographical groups of the new identified genotypes were determined through an analysis of genetic diversity. The presence of grapevine fanleaf virus, grapevine fleck virus, and grapevine leafroll-associated viruses was also determined due to the requirement of healthy clones of the new varieties for their potential interest to be authorized for cultivation in Spain.

Funder

Conservation of Phytogenetic Resources of Vine

Research and Technological Innovation in Viticulture

Publisher

MDPI AG

Subject

Horticulture,Plant Science

Reference45 articles.

1. Clemente y Rubio, S.R. (1807). Ensayo Sobre las Variedades de la vid Común que Vegetan en Andalucía: Con un índice Etimológico y Tres Listas de Plantas en que se Ca-Racterizan Varias Especies Nuevas, Imprenta de Villalpando.

2. García de los Salmones, N. (1912, January 11–22). Memoria general de las sesiones del Congreso y ponencias presentadas. Proceedings of the Congreso Nacional de Viticultura, Pamplona, Spain.

3. Rodríguez-Torres, I., Márquez, M.I., Ramírez, M.P., and Cretazzo, E. (2021, January 17–21). Caracterización molecular e identificación de variedades minoritarias de vid de cultivo tradicional en Andalucía. Proceedings of the Congreso Nacional de Ciencias Hortícolas, Córdoba, Spain.

4. OIV (2017). Distribution of the World’s Grapevine Varieties, OIV. Available online: https://www.oiv.int/public/me4d66ias/5distribution-of-the-worlds-grapevine-varieties.pdf.

5. Incidence of virus infection in old vineyards of local grapevine varieties from Majorca: Implications for clonal selection strategies;Cretazzo;Span. J. Agric. Res.,2010

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