Immobilization of Horseradish Peroxidase onto Montmorillonite/Glucosamine–Chitosan Composite for Electrochemical Biosensing of Polyphenols

Author:

Piccoli María Belén1,Gulotta Florencia Alejandra2,Montenegro Mariana Angélica3,Vanden Braber Noelia Luciana3ORCID,Paz Zanini Verónica Irene4,Ferreyra Nancy Fabiana1

Affiliation:

1. Instituto de Investigaciones en Fisicoquímica de Córdoba (INFIQC-UNC-CONICET), Departamento de Fisicoquímica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba X5000HUA, Argentina

2. Independent Researcher, Santiago del Estero G4206XCP, Argentina

3. Centro de Investigaciones y Transferencia de Villa María (CIT-VM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Villa María (UNVM), Villa María X5220XAO, Argentina

4. Instituto de Bionanotecnología del NOA (INBIONATEC), Consejo Nacional de Investigaciones Científicas y Técnicas CONICET, Universidad Nacional de Santiago del Estero (UNSE), Santiago del Estero G4206XCP, Argentina

Abstract

Glucosamine–chitosan synthesized by the Maillard reaction was combined with montmorillonite to obtain a nanohybrid composite to immobilize horseradish peroxidase. The material combines the advantageous properties of clay with those of the chitosan derivative; has improved water solubility and reduced molecular weight and viscosity; involves an eco-friendly synthesis; and exhibits ion exchange capacity, good adhesiveness, and a large specific surface area for enzyme adsorption. The physicochemical characteristics of the composite were analyzed by infrared spectroscopy and X-ray diffraction to determine clay–polycation interactions. The electrochemical response of the different polyphenols to glassy carbon electrodes modified with the composite was evaluated by cyclic voltammetry. The sensitivity and detection limit values obtained with the biosensor toward hydroquinone, chlorogenic acid, catechol, and resorcinol are (1.6 ± 0.2) × 102 µA mM−1 and (74 ± 8) nM; (1.2 ± 0.1) × 102 µA mM−1 and (26 ± 3) nM; (16 ± 2) µA mM−1 and (0.74 ± 0.09) μM; and (3.7± 0.3) µA mM−1 and (3.3 ± 0.2) μM, respectively. The biosensor was applied to quantify polyphenols in pennyroyal and lemon verbena extracts.

Funder

Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Agencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación:

Universidad Nacional de Córdoba

Universidad Nacional de Santiago del Estero

Publisher

MDPI AG

Reference57 articles.

1. Extraction, Characterization of Polyphenols from Certain Medicinal Plants and Evaluation of Their Antioxidant, Antitumor, Antidiabetic, Antimicrobial Properties, and Potential Use in Human Nutrition;Mueed;Front. Nutr.,2023

2. UV-Visible Spectroscopic Technique for Prediction of Total Polyphenol Contents for a Bunch of Medicinal Plant Extracts;Guemari;Res. Sq.,2022

3. Identification and Quantification of Polyphenols from Pineapple Peel by High Performance Liquid Chromatography Analysis;Lubaina;Adv. Zool. Bot.,2020

4. Determination of the Polyphenolic Content of Berry Juices Using Focusing-Modulated Comprehensive Two-Dimensional Liquid Chromatography Coupled to Mass Spectrometry Detection;Arena;Anal. Bioanal. Chem.,2023

5. Electrochemical and Spectrophotometric Methods for Polyphenol and Ascorbic Acid Determination in Fruit and Vegetable Extracts;Tulli;Food Technol. Biotechnol.,2020

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3