Abstract
Glutamate is the major excitatory neurotransmitter in the central nervous system, and there is evidence that Group-I metabotropic glutamate receptors (mGlu1 and mGlu5) have established roles in excitatory neurotransmission and synaptic plasticity. While glutamate is abundantly present in the gut, it plays a smaller role in neurotransmission in the enteric nervous system. In this study, we examined the roles of Group-I mGlu receptors in gastrointestinal function. We investigated the expression of Grm1 (mGlu1) and Grm5 (mGlu5) in the mouse myenteric plexus using RNAscope in situ hybridization. Live calcium imaging and motility analysis were performed on ex vivo preparations of the mouse colon. mGlu5 was found to play a role in excitatory enteric neurotransmission, as electrically-evoked calcium transients were sensitive to the mGlu5 antagonist MPEP. However, inhibition of mGlu5 activity did not affect colonic motor complexes (CMCs). Instead, inhibition of mGlu1 using BAY 36-7620 reduced CMC frequency but did not affect enteric neurotransmission. These data highlight complex roles for Group-I mGlu receptors in myenteric neuron activity and colonic function.
Funder
NHMRC Australia
NHMRC Principal Research Fellowship
NHMRC Boosting Dementia Research Leadership Fellow
ARC DECRA fellowship
ARC DECRA
Subject
Molecular Biology,Biochemistry
Reference58 articles.
1. Anderson, S.A., and Raiten, D.J. (1992). Safety of Amino Acids Used as Dietary Supplements, Federation of American Societies for Experimental Biology.
2. Consensus meeting: Monosodium glutamate—An update;Beyreuther;Eur. J. Clin. Nutr.,2007
3. Dicks, L.M.T. (2022). Gut Bacteria and Neurotransmitters. Microorganisms, 10.
4. Intestinal glutamate metabolism;Reeds;J. Nutr.,2000
5. The Roles of Dietary Glutamate in the Intestine;Ann. Nutr. Metab.,2018
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