Affiliation:
1. College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2. Key Laboratory of Integrated Pest Management in Ecological Forests, Fujian Agriculture and Forestry University, Fuzhou 350002, China
3. Laboratory of Forest Symbiology, Asian Research Center for Bioresource and Environmental Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 188-0002, Japan
4. Department of Microbiology & Molecular Genetics, University of California Irvine, Irvine, CA 92697, USA
Abstract
Monochamus alternatus Hope (Coleoptera: Cerambycidae), an invasive beetle that has caused billions of dollars of economic losses, is a serious pest of Pinus massoniana in many Asian countries. An efficient RNAi system is helpful for functional genomics research on M. alternatus. In this study, a tryptophan 2,3-dioxygenase (TDO) related to the ommochrome synthesis in insects was identified. Using RNAi technology, the M. alternatus TDO gene was silenced by injecting dsRNA into pupae, and individuals were analyzed by phenotype and expression of the TDO gene by RT-qPCR. The results show that TDO is expressed in different developmental stages of M. alternatus, having its peak expression during the prepupal stage. White-eye phenotypes were observed in the pupal and adult stages after dsRNA injection, and a significant 81% decrease in TDO mRNA levels 48 h after injection was determined by RT-qPCR. This gene can be used as a genetic marker and is an important discovery for future genetic engineering tools to control M. alternatus populations.
Funder
National Major Emergency Science and Technology Program of China
National Natural Science Foundation of China
National Key R & D Program of China
Forestry Key Program of Science and Technology in Fujian Province
Natural Science Foundation of Fujian Province, China
Forestry Programs of Science and Technology in Fujian Province
Science and Technology Program of Fujian Province
Forestry Science Research Project of Fujian Forestry Department
Forestry Peak Discipline Construction Project of Fujian Agriculture and Forestry University
Forest Science Peak Project of College of Forestry, Fujian Agriculture and Forestry University
Undergraduate Training Program for Innovation and Entrepreneurship of China
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