Genomic Characterization of a Wild-Type Bovine alphaherpesvirus 1 (BoAHV-1) Strain Isolated in an Outbreak in Central Italy-Reference-Cited by-同舟云学术

Genomic Characterization of a Wild-Type Bovine alphaherpesvirus 1 (BoAHV-1) Strain Isolated in an Outbreak in Central Italy

Published:2024-01-19 Issue:1 Volume:16 Page:150
ISSN:1999-4915
Container-title:Viruses
language:en
Short-container-title:Viruses

Author:

Petrini Stefano¹^ORCID,Curini Valentina²^ORCID,Righi Cecilia¹^ORCID,Cammà Cesare²^ORCID,Di Lollo Valeria²,Tinelli Elena¹,Mincarelli Luana Fiorella²^ORCID,Rossi Elisabetta¹^ORCID,Costantino Giulia¹^ORCID,Secondini Barbara²,Pirani Silvia¹^ORCID,Giammarioli Monica¹^ORCID,Feliziani Francesco¹^ORCID

Affiliation:

1. National Reference Centre for Infectious Bovine Rhinotracheitis (IBR), Istituto Zooprofilattico Sperimentale Umbria-Marche “Togo Rosati”, 06126 Perugia, Italy

2. National Reference Center for Whole Genome Sequencing of Microbial Pathogens, Istituto Zooprofilattico Sperimentale Abruzzo-Molise “G. Caporale”, 64100 Teramo, Italy

Abstract

Bovine alphaherpesvirus-1 (BoAHV-1) infection is common in cattle worldwide. However, information on the spread of BoAHV-1-circulating strains in Italy remains limited. In this study, we investigated an outbreak characterized by severe respiratory symptoms in a cattle herd (n = 30) located in Central Italy. BoAHV-1 was isolated from three cattle in a cell culture, which confirmed viral infection. Next, we characterized one (16453/07 TN) of the three isolates of BoAHV-1 using whole-genome sequencing. BLASTn and phylogenetic analysis revealed a nucleotide identity >99% with all BoAHV-1 strains belonging to subtype 1.1, highlighting the genetic stability of the virus. This study reports the first full genomic characterization of a BoAHV-1 isolate in Italy, enriching our understanding of the genetic characteristics of the circulating BoAHV-1 strain in Italy.

Funder

Istituto Zooprofilattico Sperimentale Umbria-Marche in Perugia, Italy

Publisher

MDPI AG

Link

https://www.mdpi.com/1999-4915/16/1/150/pdf

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