Highly Conserved Microchromosomal Organization in Passeriformes Birds Revealed via BAC-FISH Analysis
Author:
de Souza Marcelo Santos1ORCID, Barcellos Suziane Alves1ORCID, Tura Victoria1ORCID, Bobrowski Vera Lúcia2, Garnero Analía Del Valle1, Gunski Ricardo José1ORCID, Griffin Darren K.3ORCID, Kretschmer Rafael2ORCID
Affiliation:
1. Programa de Pós-Graduação em Ciências Biológicas (PPGCB), Universidade Federal do Pampa, São Gabriel 97300-000, RS, Brazil 2. Departamento de Ecologia, Zoologia e Genética, Instituto de Biologia, Universidade Federal de Pelotas, Pelotas 96010-900, RS, Brazil 3. School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK
Abstract
Passeriformes birds are widely recognized for their remarkable diversity, with over 5700 species described so far. Like most bird species, they possess a karyotype characteristic of modern birds, which includes a bimodal karyotype consisting of a few pairs of macrochromosomes and many pairs of microchromosomes. Although the karyotype is typically 2n = 80, the diploid number can atypically vary greatly, ranging from 56 to approximately 100 chromosomes. In this study, we aimed to understand the extent of conservation of the karyotype’s organizational structure within four species of this group using Bacterial Artificial Chromosomes via Fluorescence In Situ Hybridization (BAC-FISH) with microchromosome probes from Chicken (Gallus gallus) or Zebra Finch (Taeniopygia guttata) per microchromosomes (GGA10-28, except GGA16). By examining the chromosome complement of four passerine species—the Streaked Flycatcher (Myiodynastes maculatus), Shiny Cowbird (Molothrus bonariensis), Southern House Wren (Troglodytes aedon), and Double-collared Seedeater (Sporophila caerulescens)—we discovered a new chromosome number for Southern House Wren. Through FISH experiments, we were able to observe the same pattern of microchromosome organization as in the common ancestor of birds. As a result, we propose a new diploid number for Southern House Wren and confirm the conservation status of microchromosome organization, which may confer evolutionary advantages to this group.
Funder
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior–Brasil Conselho Nacional de Desenvolvimento Científico e Tecnológico
Subject
Process Chemistry and Technology,Economic Geology,Fuel Technology
Reference44 articles.
1. Sibley, C.G., and Monroe, B.L. (1990). Distribution and Taxonomy of Birds of the World, Yale University Press. 2. Cracraft, J., Barker, F.K., Hedges, S.B., and Kumar, S. (2009). The Timetree of Life, Oxford University Press. 3. Chromosome-level assembly reveals extensive rearrangement in saker falcon and budgerigar, but not ostrich, genomes;Joseph;Genome Biol.,2018 4. John, B. (1990). Animal Cytogenetics, Gebrüder Borntraeger. [4th ed.]. 5. Extensive chromosomal repatterning in two congeneric species: Pytilia melba, L. and Pytilia phoenicoptera Swainson (Estrildidae; Aves);Christidis;Cytogenet. Genome Res.,1983
|
|