Ectomycorrhizal Colonisation in Declining Oak Stands on the Krotoszyn Plateau, Poland

Abstract

We describe the ectomycorrhizal (ECM) root tips and the diversity of mycorrhizal fungal species at three English oak (Quercus robur) sites (two 120 year old sites and one 60 year old site). The three oak stands in decline, located in western Poland, were characterized by a low degree of vital ECM colonization: 30.2%, 29.1% and 25.6% at Krotoszyn (K), Piaski (P) and Karczma Borowa (KB), respectively. DNA (ITS) barcoding revealed a total of 18 ECM fungal species. Based on exploration types, ectomycorrhizae were classified with respect to ecologically relevant features. The contact type was significantly correlated with C:N and Corg, while the short distance type was correlated with Ca, phosphorus (P2O5) and pH. The medium distance exploration type was significantly correlated with fine-grained soil particle size fractions: coarse silt (0.05–0.02 mm) and fine silt (0.02–0.002 mm), and clay (<0.002 mm). The long distance type showed a similar pattern to the medium distance smooth type, but was also correlated with nitrate (N). The values of biometric root parameters of oak trees at the analysed forest sites were arranged as follows: K > P > KB, and were opposite to the condition of the tree crowns. A negative correlation of vital ECM root tip abundance with the crown health status of oaks was observed, whereas higher ECM diversity reflected better crown health in the oak stands studied.