Development and Application of Real-Time PCR-Based Screening for Identification of Omicron SARS-CoV-2 Variant Sublineages
Author:
Esman Anna1ORCID, Dubodelov Dmitry1ORCID, Khafizov Kamil12ORCID, Kotov Ivan12ORCID, Roev German12ORCID, Golubeva Anna1ORCID, Gasanov Gasan1ORCID, Korabelnikova Marina1ORCID, Turashev Askar1ORCID, Cherkashin Evgeniy1ORCID, Mironov Konstantin1ORCID, Cherkashina Anna1ORCID, Akimkin Vasily1ORCID
Affiliation:
1. Central Research Institute of Epidemiology, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing Russian Federation, 3a Novogireevskaya Str., 111123 Moscow, Russia 2. Moscow Institute of Physics and Technology, National Research University, 115184 Dolgoprudny, Russia
Abstract
The Omicron strain is currently the main dominant variant of SARS-CoV-2, with a large number of sublineages. In this article, we present our experience in tracing it in Russia using molecular diagnostic methods. For this purpose, different approaches were used; for example, we developed multiprimer panels for RT-PCR and Sanger and NGS sequencing methods. For the centralized collection and analysis of samples, the VGARus database was developed, which currently includes more than 300,000 viral sequences.
Subject
Genetics (clinical),Genetics
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