Stacking Multiple Genes Improves Resistance to Chilo suppressalis, Magnaporthe oryzae, and Nilaparvata lugens in Transgenic Rice
Author:
Li Bai1ORCID,
Chen Zhongkai1ORCID,
Chen Huizhen2,
Wang Chunlei1,
Song Liyan1,
Sun Yue1,
Cai Yicong1,
Zhou Dahu1,
Ouyang Linjuan1,
Zhu Changlan1,
He Haohua1,
Peng Xiaosong1
Affiliation:
1. Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Research Center of Super Rice Engineering and Technology, Jiangxi Agriculture University, Nanchang 330045, China
2. Pingxiang Center for Agricultural Sciences and Technology Research, Nanchang 330200, China
Abstract
The ability of various pests and diseases to adapt to a single plant resistance gene over time leads to loss of resistance in transgenic rice. Therefore, introduction of different pest and disease resistance genes is critical for successful cultivation of transgenic rice strains with broad-spectrum resistance to multiple pathogens. Here, we produced resistance rice lines with multiple, stacked resistance genes by stacking breeding and comprehensively evaluated their resistance to Chilo suppressalis (striped rice stemborer), Magnaporthe oryzae (rice blast), and Nilaparvata lugens (brown planthopper) in a pesticide-free environment. CRY1C and CRY2A are exogenous genes from Bacillus thuringiensis. Pib, Pikm, and Bph29 are natural genes in rice. CH121TJH was introduced into CRY 1C, Pib, Pikm, and Bph29. CH891TJH and R205XTJH were introduced into CRY 2A, Pib, Pikm, and Bph29. Compared with those observed in their recurrent parents, CH121TJH significantly increased the mortality of borers. The other two lines CH891TJH and R205XTJH are the same result. Three lines introduction of Pib and Pikm significantly reduced the area of rice blast lesions, and introduction of Bph29 significantly reduced seedling mortality from N. lugens. Introduction of the exogenous genes had relatively few effects on agronomic and yield traits of the original parents. These findings suggest that stacking of rice resistance genes through molecular marker-assisted backcross breeding can confer broad spectrum and multiple resistance in differently genetic backgrounds.
Funder
Major Project of Jiangxi Provincial Department of Science and Technology
Breeding New Varieties of the National Transgene of China
Jiangxi Provincial Natural Science Foundation of China
District Science Foundation Project of National Natural Science Foundation
Jiangxi Natural Science Foundation
The Major Project of Jiangxi Provincial Department of Science and Technology
Subject
Genetics (clinical),Genetics