Shotgun Metagenomic Sequencing Reveals Virome Composition of Mosquitoes from a Transition Ecosystem of North-Northeast Brazil

Author:

Aragão Carine Fortes1ORCID,da Silva Sandro Patroca1,do Nascimento Bruna Laís Sena1ORCID,da Silva Fábio Silva1,Nunes Neto Joaquim Pinto1ORCID,Pinheiro Valéria Cristina Soares2,Cruz Ana Cecília Ribeiro1ORCID

Affiliation:

1. Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil

2. Laboratory of Medical Entomology, Stadual University of Maranhão, Caxias 65604-380, MA, Brazil

Abstract

A wide diversity of pathogenic mosquito-borne viruses circulate in the Brazilian Amazon, and the intense deforestation can contribute to the spread of these viruses. In this context, this study aimed to investigate the viral diversity in mosquitoes of the genera Aedes, Culex, Haemagogus, and Sabethes from a transition area between the Amazon, Cerrado, and Caatinga biomes in Brazil. Metagenomic high-throughput sequencing was used to characterize the virome of 20 mosquito pools. A total of 15 virus-like genomes were identified, comprising species genomically close to insect-specific viruses of the families Iflaviridae, Metaviridae, Lispiviridae, Rhabdoviridae, Xinmoviridae, and Parvoviridae and species of plant viruses of the families Solemoviridae, Virgaviridae, and Partitiviridae. However, sequences of viruses associated with human and animal diseases were not detected. Most of the recovered genomes were divergent from those previously described. These findings reveal that there are a large number of unknown viruses to be explored in the middle-north of Brazil.

Funder

Programa Nacional de Cooperação Acadêmica na Amazônia

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

Conselho Nacional de Desenvolvimento Científico e Tecnológico

Evandro Chagas Institute, Stadual University of Maranhão

Fundação de Amparo à Pesquisa e Desenvolvimento Científico e Tecnológico do Maranhão

Publisher

MDPI AG

Subject

Genetics (clinical),Genetics

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