Identification of Key Genes Regulating Sorghum Mesocotyl Elongation through Transcriptome Analysis
Author:
Ju Lan1, Lv Na2, Yin Feng2, Niu Hao1, Yan Haisheng1, Wang Yubin1, Fan Fangfang1, Lv Xin1, Chu Jianqiang1, Ping Junai1
Affiliation:
1. Shanxi Key Laboratory of Sorghum Genetic and Germplasm Innovation, Sorghum Research Institute, Shanxi Agricultural University, Jinzhong 030600, China 2. College of Agriculture, Shanxi Agricultural University, Jinzhong 030600, China
Abstract
Sorghum with longer mesocotyls is beneficialfor improving its deep tolerance, which is important for the seedling rates. Here, we perform transcriptome analysis between four different sorghum lines, with the aim of identifying the key genes regulating sorghum mesocotyl elongation. According to the mesocotyl length (ML) data, we constructed four comparison groups for the transcriptome analysis and detected 2705 common DEGs. GO and KEGG enrichment analysis showed that the most common category of DEGs were involved in cell wall, microtubule, cell cycle, phytohormone, and energy metabolism-related pathways. In the cell wall biological processes, the expression of SbEXPA9-1, SbEXPA9-2, SbXTH25, SbXTH8-1, and SbXTH27 are increased in the sorghum lines with long ML. In the plant hormone signaling pathway, five auxin-responsive genes and eight cytokinin/zeatin/abscisic acid/salicylic acid-related genes showed a higher expression level in the long ML sorghum lines. In addition, five ERF genes showed a higher expression level in the sorghum lines with long ML, whereas two ERF genes showed a lower expression level in these lines. Furthermore, the expression levels of these genes were further analyzed using real-time PCR (RT-qPCR), which showed similar results. This work identified the candidate gene regulating ML, which may provide additional evidence to understand the regulatory molecular mechanisms of sorghum mesocotyl elongation.
Funder
Shanxi Province Excellent Doctoral Work Award-Scientific Research Project Hou Ji Laboratory Shanxi Province Science Foundation for Youths China Agriculture Research System of MOF and MARA Natural Science Foundation of China Sorghum Research Institute of Shanxi Agricultural University Breeding Foundation for National Natural Science Foundation of China
Subject
Genetics (clinical),Genetics
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