Affiliation:
1. State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China
2. Institute of Oceanography, College of Geography and Oceanography, Minjiang University, Fuzhou 350108, China
3. State Key Laboratory of Mariculture Breeding, Xiamen University, Xiamen 361102, China
Abstract
Bone morphogenetic proteins (BMPs) play important roles in a lot of biological processes, such as bone development, cell proliferation, cell differentiation, growth, etc. However, the functions of abalone BMP genes are still unknown. This study aimed to better understand the characterization and biological function of BMP7 of Haliotis discus hannai (hdh-BMP7) via cloning and sequencing analysis. The coding sequence (CDS) length of hdh-BMP7 is 1251 bp, which encodes 416 amino acids including a signal peptide (1–28 aa), a transforming growth factor-β (TGF-β) propeptide (38–272 aa), and a mature TGF-β peptide (314–416 aa). The analysis of expression showed that hdh-BMP7 mRNA was widely expressed in all the examined tissues of H. discus hannai. Four SNPs were related to growth traits. The results of RNA interference (RNAi) showed that the mRNA expression levels of hdh-BMPR I, hdh-BMPR II, hdh-smad1, and hdh-MHC declined after hdh-BMP7 was silenced. After RNAi experiment for 30 days, the shell length, shell width, and total weight were found to be reduced in H. discus hannai (p < 0.05). The results of real-time quantitative reverse transcription PCR revealed that the hdh-BMP7 mRNA was lower in abalone of the S-DD-group than in the L-DD-group. Based on these data, we hypothesized that BMP7 gene has a positive role in the growth of H. discus hannai.
Funder
Seed Industry Innovation and Industrialization in Fujian Province
Special Fund for Ocean and Fisheries of Xiamen
Key S & T Program of Fujian Province
Earmarked Fund for CARS
Fujian Provincial S & T Project
Subject
Genetics (clinical),Genetics
Cited by
1 articles.
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