Recombinant Oil-Body-Expressed Oleosin-hFGF5 in Arabidopsis thaliana Regulates Hair Growth

Abstract

FGF5 (Fibroblast Growth Factor) is a member of the fibroblast growth factor family, which not only regulates growth and development but also inhibits hair regeneration. The oil-body expression vector pOTB-hFGF5 was constructed by the genetic engineering method and it was transformed into Arabidopsis by flora dip. T3 homozygous transgenic Arabidopsis was obtained after screening and propagation by the PCR and Western blot methods. The recombinant oil-body-expressed oleosin-hFGF5 can inhibit the proliferation of hair follicle epithelial cells and it exhibits the pharmacological activity of inhibiting hair regeneration in vivo by protein hybridization and imunohistochemistry. At the same time, the potential mechanism of recombinant oil-body-expressed oleosin-hFGF5 inhibiting hair growth was also revealed by RNA-Seq. This implies that the recombinant oil-body-expressed oleosin-hFGF5 has a good effect on inhibiting hair growth.