The Genetic Diversity and Genetic Structure of the Germplasm Resources of the Medicinal Orchid Plant Habenaria dentata
Author:
Yang Yishan1,
Tang Jianmin12ORCID,
Zou Rong1,
Luo Yajin3,
Deng Zhenhai3,
Li Dongxin1,
Chai Shengfeng1,
Wei Xiao1
Affiliation:
1. Guangxi Key Laboratory of Plant Functional Substances and Resources Sustainable Utilization, Guangxi Institute of Botany, Guilin 541006, China
2. The United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima 890-0065, Japan
3. Guangxi Yachang Orchid National Nature Reserve Management Center, Baise 533200, China
Abstract
Habenaria dentata has medicinal and ornamental value, but the number of wild populations is decreasing dramatically. Thus, conducting research on its genetic diversity and structure is necessary to provide a basis for its conservation. This study aimed to explore the genetic diversity of the wild plant H. dentata and protect and optimize its wild resources. The genetic diversity of 133 samples from six wild populations of H. dentata was analyzed using Inter Simple Sequence Repeat molecular markers to provide a scientific basis for the screening of improved germplasm resources. The results showed that the average number of alleles was 1.765, the average number of effective alleles was 1.424, the average Nei’s gene diversity index was 0.252, the average Shannon diversity index was 0.381, and the average percentage of polymorphic loci was 76.499%. The variation within the populations was 77.34%, and the variation between the populations was 22.66%. The gene flow was 1.705, which was greater than 1. The results of the cluster analysis showed that the six populations were mainly divided into four clusters and were not classified according to their geographical location. There was no significant correlation between the geographical location and genetic distance between the populations (r = 0.557, p > 0.05). The genetic diversity of H. dentata is high. Among the six wild populations, the genetic diversity of the Mulun population was the highest and this population can be used as a key protection unit. The study on the genetic diversity of H. dentata can not only reveal the reasons for the decrease in the number of individuals in the population to a certain extent, and put forward the protection strategy, but also provide a scientific basis for the breeding of excellent seed resources.
Funder
National Key Research and Development Program
Chinese Academy of Sciences ‘Light of West China’ Program
Guangxi Forestry Science and Technology Promotion Demonstration Project
Guangxi Key Laboratory of Plant Functional Phytochemicals Research and Sustainable Utilization Independent project
Guilin Innovation Platform and Talent Plan
Guilin City Technology Application and Promotion Plan
Subject
Genetics (clinical),Genetics
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