Improved RAPD Method for Candida parapsilosis Fingerprinting-Reference-Cited by-同舟云学术

Improved RAPD Method for Candida parapsilosis Fingerprinting

Published:2023-04-05 Issue:4 Volume:14 Page:868
ISSN:2073-4425
Container-title:Genes
language:en
Short-container-title:Genes

Author:

Wojciechowska-Koszko Iwona¹,Mnichowska-Polanowska Magdalena²,Roszkowska Paulina¹,Sławiński Michał³,Giedrys-Kalemba Stefania²,Dołęgowska Barbara⁴^ORCID,Sienkiewicz Monika⁵^ORCID,Hukowska-Szematowicz Beata⁶⁷^ORCID,Kwiatkowski Paweł¹^ORCID

Affiliation:

1. Department of Diagnostic Immunology, Pomeranian Medical University in Szczecin, 70-111 Szczecin, Poland

2. Department of Medical Microbiology, Pomeranian Medical University in Szczecin, 70-111 Szczecin, Poland

3. Department of Laboratory Diagnostics, Public Clinical Hospital No. 2 in Szczecin, 70-111 Szczecin, Poland

4. Department of Laboratory Medicine, Pomeranian Medical University in Szczecin, 70-111 Szczecin, Poland

5. Department of Pharmaceutical Microbiology and Microbiological Diagnostic, Medical University of Lodz, 90-151 Lodz, Poland

6. Institute of Biology, University of Szczecin, 71-412 Szczecin, Poland

7. Molecular Biology and Biotechnology Center, University of Szczecin, 71-412 Szczecin, Poland

Abstract

Recently, methods based on the analysis of arbitrarily amplified target sites of genome microorganisms have been extensively applied in microbiological studies, and especially in epidemiological studies. The range of their application is limited by problems with discrimination and reproducibility resulting from a lack of standardized and reliable methods of optimization. The aim of this study was to obtain optimal parameters of the Random Amplified Polymorphic DNA (RAPD) reaction by using an orthogonal array as per the Taguchi and Wu protocol, modified by Cobb and Clark for Candida parapsilosis isolates. High Simpson’s index values and low Dice coefficients obtained in this study indicated a high level of interspecies DNA polymorphism between C. parapsilosis strains, and the optimized RAPD method proved useful in the microbiological and epidemiological study.

Publisher

MDPI AG

Subject

Genetics (clinical),Genetics

Link

https://www.mdpi.com/2073-4425/14/4/868/pdf

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