Affiliation:
1. National Agri-Food Biotechnology Institute, Mohali 140308, India
2. Chitkara University, Himachal Prades, Kalujhinda, Baddi 174103, India
3. Center of Innovative and Applied Bioprocessing, Mohali 140308, India
Abstract
Colored wheat has gained enormous attention from the scientific community, but the information available on the anthocyanin biosynthetic genes is very minimal. The study involved their genome-wide identification, in silico characterization and differential expression analysis among purple, blue, black and white wheat lines. The recently released wheat genome mining putatively identified eight structural genes in the anthocyanin biosynthesis pathway with a total of 1194 isoforms. Genes showed distinct exon architecture, domain profile, regulatory elements, chromosome emplacement, tissue localization, phylogeny and synteny, indicative of their unique function. RNA sequencing of developing seeds from colored (black, blue and purple) and white wheats identified differential expressions in 97 isoforms. The F3H on group two chromosomes and F3′5′H on 1D chromosomes could be significant influencers in purple and blue color development, respectively. Apart from a role in anthocyanin biosynthesis, these putative structural genes also played an important role in light, drought, low temperature and other defense responses. The information can assist in targeted anthocyanin production in the wheat seed endosperm.
Subject
Genetics (clinical),Genetics
Reference80 articles.
1. Roles of flavonoids in plants;Samanta;Carbon N. Y.,2011
2. Flavonoid Biosynthesis. A Colorful Model for Genetics, Biochemistry, Cell Biology, and Biotechnology;Plant Physiol.,2001
3. Yadav, V., Wang, Z., Wei, C., Amo, A., Ahmed, B., Yang, X., and Zhang, X. (2020). Phenylpropanoid Pathway Engineering: An Emerging Approach towards Plant Defense. Pathogens, 9.
4. Expression of genes involved in anthocyanin biosynthesis in relation to anthocyanin, proanthocyanidin, and flavonol levels during bilberry fruit development;Jaakola;Plant Physiol.,2002
5. Anthocyanin biosynthesis in pears is regulated by a R2R3-MYB transcription factor PyMYB10;Feng;Planta,2010
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献