Detection, Characterization and Sequencing of BTV Serotypes Circulating in Cuba in 2022
Author:
Acevedo Ana María1, Postic Lydie2ORCID, Curiel Maray1, Gondard Mathilde2ORCID, Bréard Emmanuel2ORCID, Zientara Stéphan2, Vorimore Fabien3ORCID, Tran Mai-Lan3, Turpaud Mathilde2, Savini Giovanni4ORCID, Lorusso Alessio4ORCID, Marcacci Maurilia4ORCID, Vitour Damien2ORCID, Dujardin Pascal2, Perera Carmen Laura1, Díaz Cristian1, Obret Yalainne1, Sailleau Corinne2ORCID
Affiliation:
1. National Center for Animal and Plant Health (CENSA), Carretera de Tapaste y Autopista Nacional, Apartado Postal 10, San José de las Lajas, San José de las Lajas 32700, Cuba 2. ANSES/INRAE/ENVA-UPEC, UMR 1161 Virology, Laboratoire de santé animale, 14 rue Pierre et Marie Curie, 94700 Maisons Alfort, France 3. Genomics Platform IdentyPath, Laboratory for Food Safety, ANSES, 94700 Maisons-Alfort, France 4. Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, Italy
Abstract
In Cuba, despite a high sero-prevalence of bluetongue virus (BTV), circulating serotypes remain unknown. The aim of this study was to identify circulating BTV serotypes in farms throughout the western region of Cuba. Blood samples were collected from 200 young cattle and sheep between May and July 2022 for virological analyses (PCR, viral isolation and virus neutralization) and genome sequencing. The results confirmed viral circulation, with viro-prevalence of 25% for BTV. The virus was isolated from 18 blood samples and twelve BTV serotypes were identified by sequencing RT-PCR products targeting the segment 2 of the BTV genome (BTV-1, 2, 3, 6, 10, 12, 13, 17, 18, 19, 22 and 24). Finally, the full genome sequences of 17 Cuban BTV isolates were recovered using a Sequence Independent Single Primer Amplification (SISPA) approach combined to MinION Oxford Nanopore sequencing technology. All together, these results highlight the co-circulation of a wide diversity of BTV serotypes in a quite restricted area and emphasize the need for entomological and livestock surveillance, particularly in light of recent changes in the global distribution and nature of BTV infections.
Subject
Virology,Infectious Diseases
Reference44 articles.
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