The Chinese Hamster Ovary Cell-Based H9 HA Subunit Avian Influenza Vaccine Provides Complete Protection against the H9N2 Virus Challenge in Chickens
Author:
Zhu Shunfan1, Nie Zhenyu1, Che Ying2, Shu Jianhong1, Wu Sufang2, He Yulong1, Wu Youqiang2, Qian Hong2, Feng Huapeng1ORCID, Zhang Qiang2
Affiliation:
1. Department of Biopharmacy, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China 2. Zhejiang Novo Biotech Co., Ltd., Shaoxing 312366, China
Abstract
(1) Background: Avian influenza has attracted widespread attention because of its severe effect on the poultry industry and potential threat to human health. The H9N2 subtype of avian influenza viruses was the most prevalent in chickens, and there are several commercial vaccines available for the prevention of the H9N2 subtype of avian influenza viruses. However, due to the prompt antigenic drift and antigenic shift of influenza viruses, outbreaks of H9N2 viruses still continuously occur, so surveillance and vaccine updates for H9N2 subtype avian influenza viruses are particularly important. (2) Methods: In this study, we constructed a stable Chinese hamster ovary cell line (CHO) to express the H9 hemagglutinin (HA) protein of the major prevalent H9N2 strain A/chicken/Daye/DY0602/2017 with genetic engineering technology, and then a subunit H9 avian influenza vaccine was prepared using the purified HA protein with a water-in-oil adjuvant. (3) Results: The results showed that the HI antibodies significantly increased after vaccination with the H9 subunit vaccine in specific-pathogen-free (SPF) chickens with a dose–dependent potency of the immunized HA protein, and the 50 μg or more per dose HA protein could provide complete protection against the H9N2 virus challenge. (4) Conclusions: These results indicate that the CHO expression system could be a platform used to develop the subunit vaccine against H9 influenza viruses in chickens.
Funder
Zhejiang Novo Biotech Biotechnology Co., Ltd. “Pioneer” and “Leading Goose” R&D Program of Zhejiang Fundamental Research Funds of Zhejiang Sci-Tech University Open Fund of the Shaoxing Academy of Biomedicine of Zhejiang Sci-Tech University National Major Scientific and Technological Special Project
Subject
Virology,Infectious Diseases
Reference57 articles.
1. Bat-derived influenza-like viruses H17N10 and H18N11;Wu;Trends Microbiol.,2014 2. Molecular characterization, receptor binding property, and replication in chickens and mice of H9N2 avian influenza viruses isolated from chickens, peafowls, and wild birds in eastern China;Guo;Emerg. Microbes Infect.,2021 3. Tang, X., Fu, Z., and Feng, J. (2000, January 25). The prevalence and prevention of avian influenza subtype H9. Proceedings of the Tenth Symposium of the Chinese Society of Animal Husbandry and Veterinary Medicine, Avian Diseases Branch, Hangzhou, China. 4. Yan, W., Cui, H., Engelsma, M., Beerens, N., van Oers, M.M., de Jong, M.C.M., Li, X., Liu, Q., Yang, J., and Teng, Q. (2022). Molecular and Antigenic Characterization of Avian H9N2 Viruses in Southern China. Microbiol. Spectr., 10. 5. Sun, X., Belser, J.A., and Maines, T.R. (2020). Adaptation of H9N2 Influenza Viruses to Mammalian Hosts: A Review of Molecular Markers. Viruses, 12.
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