In Vitro Evaluation of Surface Roughness and Color Variation after Two Brushing Protocols with Toothpastes Containing Different Whitening Technologies

Abstract

The aim was to evaluate the effect of different whitening toothpastes on the enamel surface roughness and color variation. Twenty-four molars were sectioned and divided into eight groups (n = 3) considering the following two factors under study: toothpaste type (Colgate® Total Original, Oral B® 3D White Luxe Perfection, Curaprox® Black is White, and Signal® White Now) and brushing protocol (short- and long-term). Surface roughness was examined by atomic force microscopy (AFM), and color change (ΔE) was measured using the CIE L*a*b* system. Data were statistically analyzed using comparative parametric tests at a 5% significance level. In the short-term protocol, only the Signal® White Now toothpaste increased surface roughness (p = 0.038) compared to the Colgate® Total Original group. No significant differences (p > 0.05) were observed in surface roughness in the long-term protocol. Regarding color variation, no statistically significant differences (p > 0.05) were observed in either protocol. Overall, the whitening toothpastes did not affect enamel surface roughness or color, except for Signal® White Now, which caused increased roughness in the short-term protocol. However, all toothpastes induced a visual change in color.