Numerical Modeling of Heat and Mass Transfer during Cryopreservation Using Interval Analysis

Abstract

In the paper, the numerical analysis of heat and mass transfer proceeding in an axially symmetrical articular cartilage sample subjected to the cryopreservation process is presented. In particular, a two-dimensional (axially symmetrical) model with imprecisely defined parameters is considered. The base of the heat transfer model is given by the interval Fourier equation and supplemented by initial boundary conditions. The phenomenon of cryoprotectant transport (Me2SO) through the extracellular matrix is described by the interval mass transfer equation. The liquidus-tracking (LT) method is used to control the temperature, which avoids the formation of ice regardless of the cooling and warming rates. In the LT process, the temperature decreases/increases gradually during addition/removal of the cryoprotectant, and the articular cartilage remains on or above the liquidus line so that no ice forms, independent of the cooling/warming rate. The discussed problem is solved using the interval finite difference method with the rules of directed interval arithmetic. Examples of numerical computations are presented in the final part of the paper. The obtained results of the numerical simulation are compared with the experimental results, realized for deterministically defined parameters.