The Effect of Phenolic-Rich Extracts of Rubus fruticosus, R. ulmifolius and Morus nigra on Oxidative Stress and Caco-2 Inhibition Growth
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Published:2024-04-30
Issue:9
Volume:16
Page:1361
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ISSN:2072-6643
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Container-title:Nutrients
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language:en
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Short-container-title:Nutrients
Author:
Martins Mariana S.1, Rodrigues Márcio12ORCID, Flores-Félix José David13ORCID, Garcia-Viguera Cristina4ORCID, Moreno Diego A.4ORCID, Alves Gilberto1ORCID, Silva Luís R.156ORCID, Gonçalves Ana C.17ORCID
Affiliation:
1. CICS-UBI—Health Sciences Research Centre, University of Beira Interior, 6201-001 Covilhã, Portugal 2. Research Unit for Inland Development, Polytechnic Institute of Guarda (UDI-IPG), 6300-654 Guarda, Portugal 3. Microbiology and Genetics Department, University of Salamanca, 37007 Salamanca, Spain 4. Laboratorio de Fitoquímica y Alimentos Saludables (LabFAS), Department Food Science and Technology, CSIC, CEBAS, Campus Universitario 25, Espinardo, 30100 Murcia, Spain 5. SPRINT—Sport Physical Activity and Health Research & Innovation Center, Instituto Politécnico da Guarda, 6300-559 Guarda, Portugal 6. CERES, Department of Chemical Engineering, University of Coimbra, 3030-790 Coimbra, Portugal 7. CIBIT—Coimbra Institute for Biomedical Imaging and Translational Research, University of Coimbra, 3000-548 Coimbra, Portugal
Abstract
Currently, a clear interest has been given to berries due to their richness in active metabolites, including anthocyanins and non-coloured phenolics. Therefore, the main aim of the present work is to investigate the phenolic profile, antioxidant abilities, and antiproliferative effects on normal human dermal fibroblasts (NHDF) and human colon carcinoma cell line (Caco-2) cells of phenolic-rich extracts from three red fruits highly appreciated by consumers: two species of blackberries (Rubus fruticosus and Rubus ulmifolius) and one species of mulberry (Morus nigra). A total of 19 different phenolics were identified and quantified by HPLC-DAD-ESI/MSn and HPLC-DAD, respectively. Focusing on the biological potential of the phenolic-rich extracts, all of them revealed notable scavenging abilities. Concerning the antiproliferative properties, R. fruticosus presented a cytotoxic selectivity for Caco-2 cells compared to NHDF cells. To deeper explore the biological potential, combinations with positive controls (ascorbic acid and 5-fluorouracil) were also conducted. Finally, the obtained data are another piece of evidence that the combination of phenolic-rich extracts from natural plants with positive controls may reduce clinical therapy costs and the possible toxicity of chemical drugs.
Funder
Foundation for Science and Technology Ministry of Science, Technology and Higher Education European Social Fund Europe Union CICS-UBI Community Funds RedFruit4Health PharmaStar European Union’s Horizon 2020 research and innovation programme
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