Abstract
Pueraria lobata (wild.) Ohwi is a leguminous plant and one of the traditional Chinese herbal medicines. Its puerarin extract is widely used in the pharmaceutical industry. This study reported a chromosome-level genome assembly for P. lobata and its characteristics. The genome size was ~939.2 Mb, with a contig N50 of 29.51 Mbp. Approximately 97.82% of the assembled sequences were represented by 11 pseudochromosomes. We identified that the repetitive sequences accounted for 63.50% of the P. lobata genome. A total of 33,171 coding genes were predicted, of which 97.34% could predict the function. Compared with other species, P. lobata had 757 species-specific gene families, including 1874 genes. The genome evolution analysis revealed that P. lobata was most closely related to Glycine max and underwent two whole-genome duplication (WGD) events. One was in a gamma event shared by the core dicotyledons at around 65 million years ago, and another was in the common ancestor shared by legume species at around 25 million years ago. The collinearity analysis showed that 61.45% of the genes (54,579 gene pairs) in G. max and P. lobata had collinearity. In this study, six unique PlUGT43 homologous genes were retrieved from the genome of P. lobata, and no 2-hydroxyisoflavanone 8-C-glucoside was found in the metabolites. This also revealed that the puerarin synthesis was mainly from the glycation of daidzein. The combined transcriptome and metabolome analysis suggested that two bHLHs, six MYBs and four WRKYs were involved in the expression regulation of puerarin synthesis structural genes. The genetic information obtained in this study provided novel insights into the biological evolution of P. lobata and leguminous species, and it laid the foundation for further exploring the regulatory mechanism of puerarin synthesis.
Funder
Science and Technology Department of Hubei Province
Horizontal science and technology of Enshi Se-De Bioengineering Co., Ltd.
Subject
Molecular Biology,Biochemistry
Reference43 articles.
1. Pueraria montana var. lobata root extract inhibits photoaging on skin through Nrf2 pathway;J. Microbiol. Biotechnol.,2019
2. Adolfo, L.M., Rao, X., and Dixon, R.A. (2022). Identification of Pueraria spp. through DNA barcoding and comparative transcriptomics. BMC Plant Biol., 22.
3. Wang, Y. (2020). Study on the Detection and Efficacy of Isoflavones Components in Pueraria in Different Regions in China. [Master’s Thesis, Beijing University of Technology].
4. Molecular characterization of the C-glucosylation for puerarin biosynthesis in Pueraria lobata;Plant J.,2017
5. Molecular cloning and functional characterization of two divergent 4-coumarate: Coenzyme A ligases from Kudzu (Pueraria lobata);Biol. Pharm. Bull.,2014