Bone Sialoprotein Immobilized in Collagen Type I Enhances Angiogenesis In Vitro and In Ovo

Author:

Kriegel Anja1,Langendorf Eva1,Kottmann Valentina1,Kämmerer Peer W.2ORCID,Armbruster Franz Paul3,Wiesmann-Imilowski Nadine24ORCID,Baranowski Andreas1ORCID,Gercek Erol1,Drees Philipp1,Rommens Pol Maria1ORCID,Ritz Ulrike1ORCID

Affiliation:

1. Department of Orthopedics and Traumatology, BiomaTiCS, University Medical Center of the Johannes Gutenberg University, 55131 Mainz, Germany

2. Department of Oral- and Maxillofacial Surgery, BiomaTiCS, University Medical Center Mainz, 55131 Mainz, Germany

3. Immundiagnostik AG, 64673 Bensheim, Germany

4. Department of Otorhinolaryngology, BiomaTiCS, University Medical Center Mainz, 55131 Mainz, Germany

Abstract

Bone fracture healing is a multistep process, including early immunological reactions, osteogenesis, and as a key factor, angiogenesis. Molecules inducing osteogenesis as well as angiogenesis are rare, but hold promise to be employed in bone tissue engineering. It has been demonstrated that the bone sialoprotein (BSP) can induce bone formation when immobilized in collagen type I, but its effect on angiogenesis still has to be characterized in detail. Therefore, the aim of this study was to analyse the effects of BSP immobilized in a collagen type I gel on angiogenesis. First, in vitro analyses with endothelial cells (HUVECs) were performed detecting enhancing effects of BSP on proliferation and gene expression of endothelial markers. A spheroid model was employed confirming these results. Finally, the inducing impact of BSP-collagen on vascular density was proved in a yolk sac membrane assay. Our results demonstrate that BSP is capable of inducing angiogenesis and confirm that collagen type I is the optimal carrier for this protein. Taking into account former results, and literature showing that BSP also induces osteogenesis, one can hypothesize that BSP couples angiogenesis and osteogenesis, making it a promising molecule to be used in bone tissue regeneration.

Funder

Immundiagnostik AG, Bensheim, Germany

Publisher

MDPI AG

Subject

Polymers and Plastics,General Chemistry

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