Construction and Performance Evaluation of Nicandra physalodes (Linn.) Gaertn. Polysaccharide-Based Nanogel

Abstract

The nanogels made from these polysaccharides and their derivatives are often used to construct drug delivery systems owing to their biocompatible, biodegradable, non-toxic, water-soluble, and bioactive characteristics. In this work, a novel pectin with unique gelling properties was extracted from the seed of Nicandra physalodes (NPGP). The structural research indicated that NPGP was a low methoxyl pectin with a high content of galacturonic acid. NPGP-based nanogels (NGs) were accomplished employing the water in oil (W/O) nano-emulsion method. The cysteamine containing reduction-responsive bond and integrin-targeting RGD peptide were also grafted onto NPGP. The anti-tumor drug doxorubicin hydrochloride (DOX) was loaded during the formation of NGs, and the performance of DOX delivery was studied. The NGs were characterized by UV-vis, DLS, TEM, FT-IR, and XPS. The results showed that the prepared NGs were nanosized (167.6 ± 53.86 nm), had excellent encapsulation efficiency (91.61 ± 0.85%), and possessed a fine drug loading capacity (8.40 ± 0.16%). The drug release experiment showed that DOX@NPGP-SS-RGD had good redox-responsive performance. Furthermore, the results of cell experiments revealed good biocompatibility of prepared NGs, along with selective absorption by HCT-116 cells through integrin receptor-mediated endocytosis to play an anti-tumor effect. These studies indicated the potential application of NPGP-based NGs as targeted drug delivery systems.