Dietary Supplementation with R-(+)-Limonene Improves Growth, Metabolism, Stress, and Antioxidant Responses of Silver Catfish Uninfected and Infected with Aeromonas hydrophila

Author:

Da Silva Elisia Gomes1ORCID,Finamor Isabela Andres1,Bressan Caroline Azzolin1,Schoenau William1,Vencato Marina De Souza2,Pavanato Maria Amália1,Cargnelutti Juliana Felipetto3,Da Costa Sílvio Teixeira2,Antoniazzi Alfredo Quites1ORCID,Baldisserotto Bernardo1ORCID

Affiliation:

1. Department of Physiology and Pharmacology, Universidade Federal de Santa Maria, Santa Maria 97105-900, RS, Brazil

2. Department of Morphology, Universidade Federal de Santa Maria, Santa Maria 97105-900, RS, Brazil

3. Department of Preventive Veterinary Medicine, Universidade Federal de Santa Maria, Santa Maria 97105-900, RS, Brazil

Abstract

R-(+)-limonene is a monoterpene from plants of the genus Citrus with diverse biological properties. This research evaluated the effects of dietary supplementation with R-(+)-limonene on growth, metabolic parameters in plasma and liver, and the antioxidant and stress responses in silver catfish, Rhamdia quelen, challenged or not with Aeromonas hydrophila. Fish were fed for 67 days with different doses of R-(+)-limonene in the diet (control 0.0, L0.5, L1.0, and L2.0 mL/kg of diet). On the 60th day, a challenge with A. hydrophila was performed. R-(+)-limonene in the diet potentiated the productive performance of the fish. The metabolic and antioxidant responses indicate that R-(+)-limonene did not harm the health of the animals and made them more resistant to the bacterial challenge. Histological findings showed the hepatoprotective effect of dietary R-(+)-limonene against A. hydrophila. Igf1 mRNA levels were upregulated in the liver of fish fed with an L2.0 diet but downregulated with bacterial challenge. The expression levels of crh mRNA were higher in the brains of fish fed with the L2.0 diet. However, the L2.0 diet downregulated crh and hspa12a mRNA expression in the brains of infected fish. In conclusion, the results indicated that R-(+)-limonene can be considered a good dietary supplement for silver catfish.

Funder

Nacional de Desenvolvimento Tecnológico

Publisher

MDPI AG

Subject

General Veterinary,Animal Science and Zoology

Reference125 articles.

1. FAO (2023, October 01). Fisheries and Aquaculture Division. Fishing and Aquaculture Statistics. Global Aquaculture Production 1950–2021 (FishStatJ). Pomegranate. Available online: https://www.fao.org/fishery/statistics-query/en/global_production/global_production_quantity.

2. Fish welfare: Current issues in aquaculture;Ashley;Appl. Anim. Behav. Sci.,2007

3. Effects of rearing conditions on behavioural responses, social kinetics and physiological parameters in gilthead sea bream Sparus aurata;Barroso;Appl. Anim. Behav. Sci.,2017

4. Pan-genome analysis of Aeromonas hydrophila, Aeromonas veronii and Aeromonas caviae indicates phylogenomic diversity and greater pathogenic potential for Aeromonas hydrophila;Ghatak;Antonie Van Leeuwenhoek,2016

5. Austin, B., and Austin, D.A. (2007). Bacterial Fish Pathogens. Disease of Farmed and Wild Fish, Springer. [4th ed.].

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3