The Identification of Selective Pathogenic Microbial Community Biofilms in Different Distribution Pipeline Materials and Their Disinfection Kinetics
Author:
Bhatti Sanam1, Gadhi Tanveer A.1, Mahar Rasool Bux2, Ali Imran3ORCID, Channa Najeebullah4, Kori Junaid Ahmed5ORCID, Bonelli Barbara4ORCID
Affiliation:
1. U.S. Pakistan Center for Advanced Studies in Water (USPCASW), Mehran University of Engineering and Tecnology, Jamshoro 76062, Pakistan 2. Benazir Bhutto Shaheed University of Technology and Skill Development Khairpur Mirs, Khairpur 66151, Pakistan 3. Department of Environmental Sciences, Sindh Madressatul Islam University, Aiwan-e-Tijarat Road, Karachi 74000, Pakistan 4. Department of Applied Science and Technology, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino, Italy 5. Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan
Abstract
Biofilms in water distribution lines strongly affect water safety as they are the main carriers of pathogens. The current study investigated the biofilm formation and identification of selected pathogens in different distribution pipeline materials and their disinfection method in an annular reactor (AR). Initially, the quality of the flowing water from each pipeline material was analyzed, i.e., pH, TDS, EC, turbidity, and salinity; then, the biofilm formation was monitored for each material, i.e., ABS, PC, PVC, PP, and HDPE. Further, the disinfection kinetics of biofilm at different chlorine doses, i.e., 0.5, 1.0, 1.5, and 2.0 mg/L, was investigated. The selected pathogens, i.e., E. coli, Pseudomonas, Shigella, Salmonella sp., and Vibrio sp. were identified in biofilms formed in different pipeline materials. The disinfection kinetics results showed that a chlorine dose of 2.0 mg/L was the most effective in disinfecting selected pathogens. Following the disinfection kinetics, it was observed that Salmonella sp. was disinfected within 7 days, whereas other pathogenic biofilms were disinfected within 14 days. The efficacy of chlorine disinfection was affected by the types of pipeline materials. The study outcomes could provide insights into biofilms’ disinfection method and the selection of suitable pipeline materials to ensure drinking water safety.
Funder
Higher Education Commission (HEC), Pakistan Center of Excellence (CoE) Grant, HEC, Pakistan
Subject
Water Science and Technology,Aquatic Science,Geography, Planning and Development,Biochemistry
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