Genetic Diversity of Aspergillus flavus on Maize in Guatemala

Author:

Weaver Mark A.1ORCID,Bowen Curt2,Park Lilly C.1,Bastidas Angela2,Drewry Samantha G.3ORCID,Mandel Jennifer R.3ORCID

Affiliation:

1. USDA ARS National Biological Control Laboratory, 59 Lee Road, Stoneville, MS 38776, USA

2. Semilla Nueva, 7 Avenida 14-44 Zona 9 Edificio La Galería, Oficina 35 Guatemala, Guatemala City 01009, Guatemala

3. Department of Biological Sciences, University of Memphis, 3774 Walker Avenue, Memphis, TN 38152, USA

Abstract

Aflatoxin contamination of maize is a leading threat to health in Guatemala. This contamination is the result of infection from Aspergillus flavus and has been effectively reduced in other countries through application of nonaflatoxigenic, indigenous strains of A. flavus. We collected 82 maize samples from throughout Guatemala in two years and isolated 272 A. flavus from these samples, including 126 unique genotypes. We provide here a phenotypic and simple sequence repeat (SSR)-based genotypic description of these isolates, as well as an analysis of the diversity of this population. High levels of genetic diversity were observed with the nonaflatoxigenic isolates in this study, but this information contributes to the development of indigenous aflatoxin biocontrol products.

Funder

Agricultural Research Service

the U.S Agency for International Development (USAID) Participating Agency Service Agreement

Publisher

MDPI AG

Subject

Plant Science,Health Professions (miscellaneous),Health (social science),Microbiology,Food Science

Reference30 articles.

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2. Ministerio de Agricultura, Ganadería y Alimentación—MAGA (2020). Plan Operativo Anual 2020—Reprogramación, MAGA.

3. Tay, K. (2023, October 16). Grain and Animal Feed. USDA Foreign Agricultural Service, GT2022-0004, Guatemala, 15 April 2022, Available online: https://apps.fas.usda.gov/newgainapi/api/Report/DownloadReportByFileName?fileName=Grain%20and%20Feed%20Annual_Guatemala%20City_Guatemala_GT2022-0004.pdf.

4. Tay, K. (2023, October 16). Grain and Feed Annual. USDA Foreign Agricultural Service, GT2023-0001, Guatemala, 6 April 2023, Available online: https://apps.fas.usda.gov/newgainapi/api/Report/DownloadReportByFileName?fileName=Grain%20and%20Feed%20Annual_Guatemala%20City_Guatemala_GT2023-0001.pdf.

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