Lavandula pedunculata Polyphenol-Rich Extracts Obtained by Conventional, MAE and UAE Methods: Exploring the Bioactive Potential and Safety for Use a Medicine Plant as Food and Nutraceutical Ingredient
Author:
Vilas-Boas Ana A.1ORCID, Goméz-García Ricardo12ORCID, Machado Manuela1ORCID, Nunes Catarina3, Ribeiro Sónia3, Nunes João3, Oliveira Ana L. S.1ORCID, Pintado Manuela1ORCID
Affiliation:
1. CBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal 2. Centro de Investigación e Innovación Científica y Tecnológica—CIICYT, Universidad Autónoma de Coahuila, Saltillo 25280, Coahuila, Mexico 3. Association BLC3—Technology and Innovation Campus, Centre Bio R&D Unit, Senhora da Conceição, 3045-155 Oliveira do Hospital, Portugal
Abstract
Nowadays, plant-based bioactive compounds (BCs) are a key focus of research, supporting sustainable food production and favored by consumers for their perceived safety and health advantages over synthetic options. Lavandula pedunculata (LP) is a Portuguese, native species relevant to the bioeconomy that can be useful as a source of natural BCs, mainly phenolic compounds. This study compared LP polyphenol-rich extracts from conventional maceration extraction (CE), microwave and ultrasound-assisted extraction (MAE and UAE). As a result, rosmarinic acid (58.68–48.27 mg/g DE) and salvianolic acid B (43.19–40.09 mg/g DE) were the most representative phenolic compounds in the LP extracts. The three methods exhibited high antioxidant activity, highlighting the ORAC (1306.0 to 1765.5 mg Trolox equivalents (TE)/g DE) results. In addition, the extracts obtained with MAE and CE showed outstanding growth inhibition for B. cereus, S. aureus, E. coli, S. enterica and P. aeruginosa (>50%, at 10 mg/mL). The MAE extract showed the lowest IC50 (0.98 mg DE/mL) for angiotensin-converting enzyme inhibition and the best results for α-glucosidase and tyrosinase inhibition (at 5 mg/mL, the inhibition was 87 and 73%, respectively). The LP polyphenol-rich extracts were also safe on caco-2 intestinal cells, and no mutagenicity was detected. The UAE had lower efficiency in obtaining LP polyphenol-rich extracts. MAE equaled CE’s efficiency, saving time and energy. LP shows potential as a sustainable raw material, allowing diverse extraction methods to safely develop health-promoting food and nutraceutical ingredients.
Funder
Ministry of Agriculture and Rural Development European Agricultural Fund for Rural Development project Nature Bioactive Food Transfer Empreende project Norte Portugal Regional Operational Programme European Regional Development Fund
Subject
Plant Science,Health Professions (miscellaneous),Health (social science),Microbiology,Food Science
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