Affiliation:
1. Department of Entomology, Nanjing Agricultural University, Nanjing 210095, China
Abstract
Global warming is posing a threat to animals. As a large group of widely distributed poikilothermal animals, insects are liable to heat stress. How insects deal with heat stress is worth highlighting. Acclimation may improve the heat tolerance of insects, but the underlying mechanism remains vague. In this study, the high temperature of 39 °C was used to select the third instar larvae of the rice leaf folder Cnaphalocrocis medinalis, an important insect pest of rice, for successive generations to establish the heat-acclimated strain (HA39). The molecular mechanism of heat acclimation was explored using this strain. The HA39 larvae showed stronger tolerance to 43 °C than the unacclimated strain (HA27) persistently reared at 27 °C. The HA39 larvae upregulated a glucose dehydrogenase gene, CmGMC10, to decrease the reactive oxygen species (ROS) level and increase the survival rate under heat stress. The HA39 larvae maintained a higher activity of antioxidases than the HA27 when confronted with an exogenous oxidant. Heat acclimation decreased the H2O2 level in larvae under heat stress which was associated with the upregulation of CmGMC10. The rice leaf folder larvae may acclimate to global warming via upregulating CmGMC10 to increase the activity of antioxidases and alleviate the oxidative damage of heat stress.
Funder
National Natural Science Foundation of China
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference67 articles.
1. Effect of environmental temperature on growth- and reproduction-related hormones gene expression in the female blue gourami (Trichogaster trichopterus);Levy;Comp. Biochem. Physiol. Part A Mol. Integr. Physiol.,2011
2. Reinhold, J.M., Lazzari, C.R., and Lahondère, C. (2018). Effects of the environmental temperature on Aedes aegypti and Aedes albopictus mosquitoes: A review. Insects, 9.
3. Shukla, P.R., Skea, J., Buendia, E.C., Masson Delmotte, V., Pörtner, H.-O., and Roberts, D.C. (2019). Climate Change and Land: An IPCC Special Report on Climate Change, Desertification, Land Degradation, Sustainable Land Management, Food Security, and Greenhouse Gas Fluxes in Terrestrial Ecosystems, Cambridge University Press.
4. Upper thermal limits in terrestrial ectotherms: How constrained are they?;Hoffmann;Funct. Ecol.,2013
5. Fertility and mortality impacts of thermal stress from experimental heatwaves on different life stages and their recovery in a model insect;Sales;R. Soc. Open Sci.,2021