Genome-Wide Analysis of Strictosidine Synthase-like Gene Family Revealed Their Response to Biotic/Abiotic Stress in Poplar
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Published:2023-06-14
Issue:12
Volume:24
Page:10117
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ISSN:1422-0067
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Container-title:International Journal of Molecular Sciences
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language:en
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Short-container-title:IJMS
Author:
Wang Ruiqi1ORCID, Zhao Wenna1, Yao Wenjing12ORCID, Wang Yuting1, Jiang Tingbo1, Liu Huanzhen1
Affiliation:
1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China 2. Co-Innovation Center for Sustainable Forestry in Southern China, Bamboo Research Institute, Nanjing Forestry University, 159 Longpan Road, Nanjing 210037, China
Abstract
The strictosidine synthase-like (SSL) gene family is a small plant immune-regulated gene family that plays a critical role in plant resistance to biotic/abiotic stresses. To date, very little has been reported on the SSL gene in plants. In this study, a total of thirteen SSLs genes were identified from poplar, and these were classified into four subgroups based on multiple sequence alignment and phylogenetic tree analysis, and members of the same subgroup were found to have similar gene structures and motifs. The results of the collinearity analysis showed that poplar SSLs had more collinear genes in the woody plants Salix purpurea and Eucalyptus grandis. The promoter analysis revealed that the promoter region of PtrSSLs contains a large number of biotic/abiotic stress response elements. Subsequently, we examined the expression patterns of PtrSSLs following drought, salt, and leaf blight stress, using RT-qPCR to validate the response of PtrSSLs to biotic/abiotic stresses. In addition, the prediction of transcription factor (TF) regulatory networks identified several TFs, such as ATMYB46, ATMYB15, AGL20, STOP1, ATWRKY65, and so on, that may be induced in the expression of PtrSSLs in response to adversity stress. In conclusion, this study provides a solid basis for a functional analysis of the SSL gene family in response to biotic/abiotic stresses in poplar.
Funder
Major Project of Agricultural Biological Breeding The Innovation Project of State Key Laboratory of Tree Genetics and Breeding
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference49 articles.
1. Phylogenetic and transcriptional analysis of a strictosidine synthase-like gene family in Arabidopsis thaliana reveals involvement in plant defence responses;Sohani;Plant Biol.,2009 2. Regulatory Gene Networks in Drought Stress Responses and Resistance in Plants;Takahashi;Adv. Exp. Med. Biol.,2018 3. Plant salt-tolerance mechanisms;Deinlein;Trends Plant Sci.,2014 4. Recent advances in the characterization of plant transcriptomes in response to drought, salinity, heat, and cold stress;Bashir;F1000Research,2019 5. Wei, H., Movahedi, A., Liu, G., Li, Y., Liu, S., Yu, C., Chen, Y., Zhong, F., and Zhang, J. (2022). Comprehensive Analysis of Carotenoid Cleavage Dioxygenases Gene Family and Its Expression in Response to Abiotic Stress in Poplar. Int. J. Mol. Sci., 23.
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